Inhibition of Vesicular stomatitis virus particle bearing EBOV glycoprotein-mediated viral entry in African green monkey Vero cells incubated for 1 hr prior to viral addition measured after 1 hr by fluorescence microscopy relative to control
Inhibition of Vesicular stomatitis virus particle bearing EBOV glycoprotein-mediated viral entry in African green monkey Vero cells at >20 uM incubated for 1 hr prior to viral addition measured after 1 hr by fluorescence microscopy relative to control
Inhibition of Vesicular stomatitis virus particle bearing EBOV glycoprotein-mediated viral entry in African green monkey Vero cells at 20 uM incubated for 1 hr prior to viral addition measured after 1 hr by fluorescence microscopy relative to control
Inhibition of Vesicular stomatitis virus particle bearing EBOV glycoprotein-mediated viral entry in African green monkey Vero cells at 75 uM incubated for 1 hr prior to viral addition measured after 1 hr by fluorescence microscopy relative to control
Inhibition of Vesicular stomatitis virus particle bearing EBOV glycoprotein-mediated viral entry in African green monkey Vero cells at 40 uM incubated for 1 hr prior to viral addition measured after 1 hr by fluorescence microscopy relative to control
Binding affinity to Ebola virus Zaire Mayinga-76 recombinant GP protein assessed as change in melting temperature at 500 uM by SYPRO orange dye-based fluorescence assay
Binding affinity to EBOV Mayinga, Zaire, 1976 envelope glycoprotein extracellular domain T42A mutant tagged with 6xHis/foldon trimerization sequence from the bacteriophage T4 fibritin at C terminal expressed in HEK293T cells assessed as decrease in melting temperature at 50 uM at pH 5.2 by SYPRO orange dye-based fluorescence assay
Binding affinity to EBOV Mayinga, Zaire, 1976 envelope glycoprotein extracellular domain H613A mutant tagged with His/foldon trimerization sequence from the bacteriophage T4 fibritin at C terminal expressed in HEK293T cells assessed as decrease in melting temperature at 500 uM at pH 5.2 by SYPRO orange dye-based fluorescence assay
Binding affinity to EBOV Mayinga, Zaire, 1976 envelope glycoprotein extracellular domain H613A mutant tagged with His/foldon trimerization sequence from the bacteriophage T4 fibritin at C terminal expressed in HEK293T cells at pH 5.2 by SYPRO orange dye-based fluorescence assay
Binding affinity to EBOV Mayinga, Zaire, 1976 envelope glycoprotein extracellular domain T42A mutant tagged with 6xHis/foldon trimerization sequence from the bacteriophage T4 fibritin at C terminal expressed in HEK293T cells at pH 5.2 by SYPRO orange dye-based fluorescence assay
Inhibition of Ebola virus Sudan envelope glycoprotein-mediated cell entry in human HuH7 cells treated with 30 mins pre-treated pseudovirus/compopund mixture and measured after 72 hrs by luciferase reporter assay