#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4007297	B	Inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using 60 ug/ml single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay	Homo sapiens	18	ALA4004889	assay format	Scientific Literature
2.	ALA4007301	B	ATP competitive inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) using varying ATP levels after 30 mins by ADP-Glo luminescence assay	Homo sapiens	1	ALA4004889	assay format	Scientific Literature
3.	ALA4007302	B	Inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) using 1.5 ug/ml single stranded poly(U) RNA after 45 mins by ADP-Glo luminescence assay	Homo sapiens	1	ALA4004889	assay format	Scientific Literature
4.	ALA4007303	B	Competitive inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) using varying levels of single stranded poly(U) RNA as substrate after 105 mins by ADP-Glo luminescence assay	Homo sapiens	1	ALA4004889	assay format	Scientific Literature
5.	ALA4007304	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) by isothermal titration calorimetric method	Homo sapiens	1	ALA4004889	assay format	Scientific Literature
6.	ALA4007305	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) administered for 60 to 120 secs by surface plasmon resonance biosensing assay	Homo sapiens	3	ALA4004889	assay format	Scientific Literature
7.	ALA4007306	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in enthalpy by isothermal titration calorimetric method	Homo sapiens	1	ALA4004889	assay format	Scientific Literature
8.	ALA4007307	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as association rate constant administered for 60 to 120 secs by surface plasmon resonance biosensing assay	Homo sapiens	3	ALA4004889	assay format	Scientific Literature
9.	ALA4007308	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation rate constant administered for 60 to 120 secs by surface plasmon resonance biosensing assay	Homo sapiens	3	ALA4004889	assay format	Scientific Literature
10.	ALA4007309	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as dissociation half-life administered for 60 to 120 secs by surface plasmon resonance biosensing assay	Homo sapiens	3	ALA4004889	assay format	Scientific Literature
11.	ALA4007310	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in Gibbs free energy by isothermal titration calorimetric method	Homo sapiens	1	ALA4004889	assay format	Scientific Literature
12.	ALA4007311	B	Binding affinity to full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as change in entropy by isothermal titration calorimetric method	Homo sapiens	1	ALA4004889	assay format	Scientific Literature
13.	ALA4007312	B	Inhibition of full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 helicase activity expressed in Escherichia coli BL21(DE3) assessed as reduction in RNA unwinding up to 300 uM using (5'-(FITC)-GCUUUACGGUGC-3')/(5'-GGGGAGAAAAACAAAACAAAACUAGCACCGUAAAGCACGC-3') RNA as substrate in presence of ATP/MLN51 after 30 mins by gel electrophoresis method	Homo sapiens	2	ALA4004889	assay format	Scientific Literature
14.	ALA4017290	B	Inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay	Homo sapiens	5	ALA4014351	assay format	Scientific Literature
15.	ALA4044705	B	Inhibition of MLN51-induced full length recombinant human N-terminal His6/SUMO-tagged eIF4A3 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay	Homo sapiens	4	ALA4043122	assay format	Scientific Literature
16.	ALA4051066	B	Inhibition of MLN51-induced full-length human recombinant His6-tagged eIF4A3 RNA dependent ATPase activity expressed in Escherichia coli BL21(DE3) using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay	Homo sapiens	37	ALA4049423	assay format	Scientific Literature
17.	ALA4051067	B	Inhibition of eIF4A3 (unknown origin) expressed in HEK293T cells assessed as NMD suppression at 10 uM after 6 hrs by luciferase reporter gene assay relative to control	Homo sapiens	7	ALA4049423	cell-based format	Scientific Literature
18.	ALA4051068	B	Inhibition of eIF4A3 (unknown origin) expressed in HEK293T cells assessed as NMD suppression at 3 uM after 6 hrs by luciferase reporter gene assay relative to control	Homo sapiens	7	ALA4049423	cell-based format	Scientific Literature
19.	ALA4051073	B	Binding affinity to full-length human recombinant His6-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) by surface plasmon resonance assay	Homo sapiens	4	ALA4049423	assay format	Scientific Literature
20.	ALA4051074	B	Binding affinity to full-length human recombinant His6-tagged eIF4A3 expressed in Escherichia coli BL21(DE3) assessed as association rate constant by surface plasmon resonance assay	Homo sapiens	3	ALA4049423	assay format	Scientific Literature