# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4020311 | B | Inhibition of Mycobacterium tuberculosis H37Rv N-terminal 6His-tagged LeuRS expressed in Escherichia coli BL21(DE3) assessed as L-[14C]leucine incorporation in to Escherichia coli tRNA after 20 mins by liquid scintillation counting analysis | Mycobacterium tuberculosis H37Rv | 44 | ALA4017537 | assay format | Scientific Literature | |
2. | ALA4374104 | B | Inhibition of Mycobacterium tuberculosis H37Rv leuRS | Mycobacterium tuberculosis H37Rv | 2 | ALA4373645 | single protein format | Scientific Literature | |
3. | ALA4374800 | B | Inhibition of Mycobacterium tuberculosis LeuRS expressed in Escherichia coli Rosetta (DE3) cells at 100 uM incubated for 30 mins followed by incubation with ATP, leucine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis relative to control | Mycobacterium tuberculosis | 37 | ALA4373683 | cell-based format | Scientific Literature | |
4. | ALA4374802 | B | Inhibition of Mycobacterium tuberculosis LeuRS expressed in Escherichia coli Rosetta (DE3) cells incubated for 30 mins followed by incubation with ATP, leucine, tRNA, iPPase for 10 mins and measured after 30 mins by BIOMOL Green reagent based plate reader analysis | Mycobacterium tuberculosis | 7 | ALA4373683 | cell-based format | Scientific Literature | |
5. | ALA4620494 | B | Inhibition of Mycobacterium tuberculosis LeuRS expressed in Escherichia coli Rosetta (DE3) cells | Mycobacterium tuberculosis | 1 | ALA4619734 | cell-based format | Scientific Literature |