| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4026607 | B | Binding affinity to 14-3-3zeta (1 to 230 residues) (unknown origin) after 1 hr using FITC labeled compound by fluorescence polarization assay | Homo sapiens | 14 | single protein format | Scientific Literature | ||
| 2. | ALA4026608 | B | Inhibition of TAMRA-labeled cRaf peptide binding to 14-3-3zeta (1 to 230 residues) (unknown origin) after 1 hr by fluorescence polarization assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 3. | ALA4026609 | B | Inhibition of 14-3-3zeta (unknown origin) in human U87 cells assessed as decrease in exogenous 14-3-3zeta-induced MMP1 mRNA levels at 20 uM after 24 hrs by RT-qPCR analysis relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 4. | ALA4026610 | B | Inhibition of 14-3-3zeta (unknown origin) in human U87 cells assessed as decrease in exogenous 14-3-3zeta-induced MMP1 mRNA levels after 24 hrs by RT-qPCR analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4028442 | B | Inhibition of PRAS40 binding to human GST-tagged 14-3-3 protein zeta/delta expressed in Escherichia coli BL21(DE3) by ELISA | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 6. | ALA4188134 | B | Binding affinity to 14-3-3zeta (unknown origin) assessed as stabilization of protein-protein interaction between 14-3-3zeta and fluorescein-labeled phosphopeptide by measuring Kd of FAM-KRSHpSV-COOH at 40 uM by fluorescence polarization assay (Rvb = 470 +/- 20 nM) | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 7. | ALA4188135 | B | Binding affinity to 14-3-3zeta (unknown origin) assessed as stabilization of protein-protein interaction between 14-3-3zeta and fluorescein-labeled phosphopeptide by measuring reduction in Kd of FAM-KRSHpSV-COOH at 40 uM by fluorescence polarization assay relative to untreated control | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 8. | ALA4229669 | B | Binding affinity to human full length 14-3-3zeta by fluorescence polarization assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 9. | ALA4229670 | B | Binding affinity to GST-tagged 14-3-3-zeta (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as inhibition of interaction with PRAS40 in African green monkey COS7 cells preincubated with protein followed by cell addition by ELISA | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4229673 | B | Binding affinity to recombinant human His-tagged 14-3-3zeta expressed in Escherichia coli K12 by ITC method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 11. | ALA4233546 | B | Binding affinity to human GST-tagged 14-3-3zeta expressed in Escherichia coli assessed as Kd for 14-3-3zeta binding to H+-ATPase peptide motif by isothermal titration calorimetric method | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 12. | ALA4270791 | B | Binding affinity to 4-3-3 protein zeta/delta in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4616350 | B | Binding affinity to 14-3-3 zeta (unknown origin) by SPR analysis | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 14. | ALA4616351 | B | Binding affinity to 14-3-3 zeta (unknown origin) assessed as 14-3-3 zeta-synaptopodin interaction by fluorescence polarization assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4616352 | B | Competitive binding affinity to 14-3-3 zeta (unknown origin) at 1000 uM in presence of 1 uM synaptopodin by SPR analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA4616353 | B | Competitive binding affinity to 14-3-3 zeta (unknown origin) at 1000 uM in presence of 25 uM synaptopodin by SPR analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 17. | ALA4616354 | B | Competitive binding affinity to 14-3-3 zeta (unknown origin) at 200 uM in presence of 1 uM synaptopodin by SPR analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 18. | ALA4616355 | B | Binding affinity to 14-3-3 zeta (unknown origin) assessed as response unit at 3.1 to 50 uM by SPR analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 19. | ALA4616356 | B | Binding affinity to 14-3-3 zeta (unknown origin) assessed as association rate constant by SPR analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 20. | ALA4616357 | B | Binding affinity to 14-3-3 zeta (unknown origin) assessed as dissociation rate constant by SPR analysis | Homo sapiens | 2 | single protein format | Scientific Literature |
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