#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4026607	B	Binding affinity to 14-3-3zeta (1 to 230 residues) (unknown origin) after 1 hr using FITC labeled compound by fluorescence polarization assay	Homo sapiens	14	ALA4024750	single protein format	Scientific Literature
2.	ALA4026608	B	Inhibition of TAMRA-labeled cRaf peptide binding to 14-3-3zeta (1 to 230 residues) (unknown origin) after 1 hr by fluorescence polarization assay	Homo sapiens	3	ALA4024750	single protein format	Scientific Literature
3.	ALA4026609	B	Inhibition of 14-3-3zeta (unknown origin) in human U87 cells assessed as decrease in exogenous 14-3-3zeta-induced MMP1 mRNA levels at 20 uM after 24 hrs by RT-qPCR analysis relative to control	Homo sapiens	3	ALA4024750	cell-based format	Scientific Literature
4.	ALA4026610	B	Inhibition of 14-3-3zeta (unknown origin) in human U87 cells assessed as decrease in exogenous 14-3-3zeta-induced MMP1 mRNA levels after 24 hrs by RT-qPCR analysis relative to control	Homo sapiens	1	ALA4024750	cell-based format	Scientific Literature
5.	ALA4028442	B	Inhibition of PRAS40 binding to human GST-tagged 14-3-3 protein zeta/delta expressed in Escherichia coli BL21(DE3) by ELISA	Homo sapiens	1	ALA4024797	assay format	Scientific Literature
6.	ALA4188134	B	Binding affinity to 14-3-3zeta (unknown origin) assessed as stabilization of protein-protein interaction between 14-3-3zeta and fluorescein-labeled phosphopeptide by measuring Kd of FAM-KRSHpSV-COOH at 40 uM by fluorescence polarization assay (Rvb = 470 +/- 20 nM)	Homo sapiens	4	ALA4184246	single protein format	Scientific Literature
7.	ALA4188135	B	Binding affinity to 14-3-3zeta (unknown origin) assessed as stabilization of protein-protein interaction between 14-3-3zeta and fluorescein-labeled phosphopeptide by measuring reduction in Kd of FAM-KRSHpSV-COOH at 40 uM by fluorescence polarization assay relative to untreated control	Homo sapiens	4	ALA4184246	single protein format	Scientific Literature
8.	ALA4229669	B	Binding affinity to human full length 14-3-3zeta by fluorescence polarization assay	Homo sapiens	2	ALA4229326	single protein format	Scientific Literature
9.	ALA4229670	B	Binding affinity to GST-tagged 14-3-3-zeta (unknown origin) expressed in Escherichia coli BL21(DE3) assessed as inhibition of interaction with PRAS40 in African green monkey COS7 cells preincubated with protein followed by cell addition by ELISA	Homo sapiens	1	ALA4229326	cell-based format	Scientific Literature
10.	ALA4229673	B	Binding affinity to recombinant human His-tagged 14-3-3zeta expressed in Escherichia coli K12 by ITC method	Homo sapiens	1	ALA4229326	assay format	Scientific Literature
11.	ALA4233546	B	Binding affinity to human GST-tagged 14-3-3zeta expressed in Escherichia coli assessed as Kd for 14-3-3zeta binding to H+-ATPase peptide motif by isothermal titration calorimetric method	Homo sapiens	1	ALA4229430	assay format	Scientific Literature
12.	ALA4270791	B	Binding affinity to 4-3-3 protein zeta/delta in human Hela cells lysates assessed as protein enrichment by measuring normalized heavy/light ratio at by nano-LC-ESIMS/MS analysis	Homo sapiens	1	ALA4270485	cell-based format	Scientific Literature
13.	ALA4616350	B	Binding affinity to 14-3-3 zeta (unknown origin) by SPR analysis	Homo sapiens	3	ALA4613321	single protein format	Scientific Literature
14.	ALA4616351	B	Binding affinity to 14-3-3 zeta (unknown origin) assessed as 14-3-3 zeta-synaptopodin interaction by fluorescence polarization assay	Homo sapiens	1	ALA4613321	single protein format	Scientific Literature
15.	ALA4616352	B	Competitive binding affinity to 14-3-3 zeta (unknown origin) at 1000 uM in presence of 1 uM synaptopodin by SPR analysis	Homo sapiens	1	ALA4613321	single protein format	Scientific Literature
16.	ALA4616353	B	Competitive binding affinity to 14-3-3 zeta (unknown origin) at 1000 uM in presence of 25 uM synaptopodin by SPR analysis	Homo sapiens	1	ALA4613321	single protein format	Scientific Literature
17.	ALA4616354	B	Competitive binding affinity to 14-3-3 zeta (unknown origin) at 200 uM in presence of 1 uM synaptopodin by SPR analysis	Homo sapiens	1	ALA4613321	single protein format	Scientific Literature
18.	ALA4616355	B	Binding affinity to 14-3-3 zeta (unknown origin) assessed as response unit at 3.1 to 50 uM by SPR analysis	Homo sapiens	2	ALA4613321	single protein format	Scientific Literature
19.	ALA4616356	B	Binding affinity to 14-3-3 zeta (unknown origin) assessed as association rate constant by SPR analysis	Homo sapiens	2	ALA4613321	single protein format	Scientific Literature
20.	ALA4616357	B	Binding affinity to 14-3-3 zeta (unknown origin) assessed as dissociation rate constant by SPR analysis	Homo sapiens	2	ALA4613321	single protein format	Scientific Literature