# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4025799 | B | Inhibition of Trypanosoma cruzi trans-sialidase at 1 mM using N-acetyllactosamine as substrate in presence of 3'-sialyllactose measured after 15 mins by HPAEC-PAD relative to control | Trypanosoma cruzi | 6 | ALA4024715 | single protein format | Scientific Literature | |
2. | ALA4156488 | B | Inhibition of Trypanosoma cruzi trans-sialidase at 1 mM assessed as reduction of 3'-sialyl-N-acetyllactosamine formation at 1 mM using N-acetyllactosamine as substrate and 3-sialyl-lactose as donor incubated for 15 mins by HPAEC-PAD relative to control | Trypanosoma cruzi | 12 | ALA4152334 | single protein format | Scientific Literature | |
3. | ALA4180003 | B | Inhibition of recombinant His-tagged Trypanosoma cruzi trans-sialidase N-terminal catalytic domain at 1 mM preincubated for 10 mins followed by MuNANA addition measured over 5 mins by fluorimetric assay relative to control | Trypanosoma cruzi | 7 | ALA4177604 | single protein format | Scientific Literature | |
4. | ALA4180005 | B | Inhibition of Trypanosoma cruzi trans-sialidase using Trifluoromethylumbelliferyl alpha-sialoside as substrate by UV/visible spectrophotometer | Trypanosoma cruzi | 1 | ALA4177604 | single protein format | Scientific Literature | |
5. | ALA4180006 | B | Inhibition of Trypanosoma cruzi trans-sialidase at 1 mM relative to control | Trypanosoma cruzi | 1 | ALA4177604 | single protein format | Scientific Literature | |
6. | ALA4267451 | B | Inhibition of recombinant HisA-tagged Trypanosoma cruzi trans-sialidase expressed in Escherichia coli BL21 (DE3) using MuNANA as substrate pretreated for 10 mins followed by substrate addition by fluorescence assay | Trypanosoma cruzi | 11 | ALA4265911 | assay format | Scientific Literature | |
7. | ALA4267456 | B | Non-competitive inhibition of recombinant HisA-tagged Trypanosoma cruzi trans-sialidase expressed in Escherichia coli BL21 (DE3) using varying levels of MuNANA as substrate by Lineweaver-burk plot analysis | Trypanosoma cruzi | 3 | ALA4265911 | assay format | Scientific Literature | |
8. | ALA4267457 | B | Inhibition of recombinant Trypanosoma cruzi trans-sialidase expressed in Escherichia coli BL21 (DE3) using MuNANA as substrate after 10 mins in presence of [D-glucose-1-14C]lactose by liquid scintillation counting method | Trypanosoma cruzi | 1 | ALA4265911 | assay format | Scientific Literature | |
9. | ALA4267458 | B | Inhibition of recombinant HisA-tagged Trypanosoma cruzi trans-sialidase expressed in Escherichia coli BL21 (DE3) using 3-sialyl-lactose as donor measured after 15 mins by HPAEC-PAD | Trypanosoma cruzi | 1 | ALA4265911 | assay format | Scientific Literature | |
10. | ALA4267459 | B | Inhibition of Trypanosoma cruzi trans-sialidase | Trypanosoma cruzi | 1 | ALA4265911 | single protein format | Scientific Literature | |
11. | ALA4267460 | B | Inhibition of Trypanosoma cruzi trans-sialidase using CF3MuSA as substrate by UV/visible spectrophotometric method | Trypanosoma cruzi | 1 | ALA4265911 | single protein format | Scientific Literature |