| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4030854 | B | Inhibition of UbcH5c (unknown origin) expressed in human 293T cells assessed as decrease in TNF-alpha-mediated NF-kB activation at 5 uM preincubated for 2 hrs followed by TNF-alpha stimulation for 8 hrs by luciferase reporter gene assay relative to control | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
| 2. | ALA4030860 | B | Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced IL-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA4030861 | B | Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MCP-1 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA4030862 | B | Inhibition of patient derived UbcH5c in synovial fibroblast cells assessed as decrease in TNF- alpha induced MMP-3 mRNA level preincubated for 2 hrs followed by TNF-alpha addition for 3 hrs by qPCR analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4030863 | B | Inhibition of UbcH5c in human HeLa cells assessed as TNF-alpha induced linear polyubiquitination of NEMO preincubated for 2 hrs followed by TNF-alpha addition for 5 mins by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4030864 | B | Binding affinity to UbcH5c in human HeLa cells up to 40 uM after 40 mins by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4030865 | B | Binding affinity to UbcH5c in human HeLa cells up to 40 uM after 40 mins by Azide-Fluor 488 dye-based fluorescence imaging assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4030870 | B | Inhibition of UbcH5c (unknown origin) at 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 15 | single protein format | Scientific Literature | ||
| 9. | ALA4030871 | B | Inhibition of UbcH5c (unknown origin) at 5 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 52 | single protein format | Scientific Literature | ||
| 10. | ALA4030872 | B | Inhibition of UbcH5c (unknown origin) at 1 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 6 | single protein format | Scientific Literature | ||
| 11. | ALA4030873 | B | Inhibition of UbcH5c (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 12. | ALA4030878 | B | Inhibition of UbcH5c (unknown origin) preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 13. | ALA4030881 | B | Binding affinity to human recombinant UbcH5c by SPR analysis | Homo sapiens | 5 | single protein format | Scientific Literature | ||
| 14. | ALA4030893 | B | Binding affinity to trypsin digested recombinant UbcH5c (unknown origin) assessed as covalent adduct formation with Cys85 residues after 1 hr by LC/MS/MS analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 15. | ALA4030894 | B | Binding affinity to trypsin digested recombinant UbcH5c (unknown origin) assessed as covalent adduct formation with Cys111 residues after 1 hr by LC/MS/MS analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 16. | ALA4030898 | B | Binding affinity to human recombinant UbcH5c assessed as association rate constant by SPR analysis | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 17. | ALA4030899 | B | Binding affinity to human recombinant UbcH5c assessed as dissociation rate constant by SPR analysis | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 18. | ALA4043718 | B | Inhibition of UbcH5c (unknown origin) assessed as RNF4 ubiquitination at 2.5 mM after 15 mins in presence of fluorescently-labeled Ub/E2 by fluorescence assay | Homo sapiens | 3 | single protein format | Scientific Literature |
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