Inhibition of Mycobacterium tuberculosis ATCC 25618 H37Rv Pks13 thioesterase domain expressed in Escherichia coli BL21(DE3) using 4-MUH as substrate by fluorescence assay
Inhibition of Mycobacterium tuberculosis H37Rv N-terminal His6-tagged Pks13 thioesterase domain expressed in Escherichia coli BL21(DE3) using 4-MUH as substrate measured at 10 mins interval over 110 mins by fluorescence assay
Inhibition of Pks13 D1644G/N1640K double mutant thioesterase domain in Mycobacterium tuberculosis H37Rv assessed as reduction in bacterial growth by microplate alamar blue assay
Inhibition of Pks13 D1644G/N1640K double mutant thioesterase domain in drug-susceptible Mycobacterium tuberculosis V4207 assessed as reduction in bacterial growth by microplate alamar blue assay
Inhibition of Pks13 D1644G/N1640K double mutant thioesterase domain in multidrug-resistant Mycobacterium tuberculosis V2475 assessed as reduction in bacterial growth by microplate alamar blue assay
Inhibition of Pks13 D1644G/N1640K double mutant thioesterase domain in multidrug-resistant Mycobacterium tuberculosis KZN494 assessed as reduction in bacterial growth by microplate alamar blue assay
Inhibition of Pks13 D1644G/N1640K double mutant thioesterase domain in extensively drug-resistant Mycobacterium tuberculosis TF274 assessed as reduction in bacterial growth by microplate alamar blue assay
Inhibition of Pks13 D1644G/N1640K double mutant thioesterase domain in extensively drug-resistant Mycobacterium tuberculosis R506 assessed as reduction in bacterial growth by microplate alamar blue assay
Inhibition of Mycobacterium tuberculosis Pks13-TE domain using 4-methylumbelliferyl heptanoate as a fluorogenic substrate measured for 110 mins at 10 mins interval
Binding affinity to Mycobacterium tuberculosis Pks13-TE N1640S mutant expressed in Escherichia coli BL21(DE3) pLysS assessed as change in melting temperature at 1:5 compound to protein ratio by nanoDSF thermal stability analysis
Binding affinity to Mycobacterium tuberculosis Pks13-TE N1640S mutant expressed in Escherichia coli BL21(DE3) pLysS assessed as change in melting temperature at 1:10 compound to protein ratio by nanoDSF thermal stability analysis
Binding affinity to Mycobacterium tuberculosis Pks13-TE N1640K mutant expressed in Escherichia coli BL21(DE3) pLysS assessed as change in melting temperature at 1:5 compound to protein ratio by nanoDSF thermal stability analysis
Binding affinity to Mycobacterium tuberculosis Pks13-TE N1640K mutant expressed in Escherichia coli BL21(DE3) pLysS assessed as change in melting temperature at 1:10 compound to protein ratio by nanoDSF thermal stability analysis