# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA3991887 | B | Kinobeads (epsilon), multiple immobilized ATP-competitive broad spectrum kinase inhibitors, used to assess residual binding of ~300 proteins simultaneously from cell lysate in the presence of a compound. Quantitative readout performed by mass spectrometry. | Homo sapiens | 241 | ALA3991601 | subcellular format | Kuster lab chemical proteomics drug profiling | |
2. | ALA4007300 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 insect cells using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 4 | ALA4004889 | cell-based format | Scientific Literature | |
3. | ALA4017286 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 cells at 3 uM using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 2 | ALA4014351 | cell-based format | Scientific Literature | |
4. | ALA4017287 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 cells at 3 uM using single stranded poly(U) RNA as substrate after 120 mins in presence of ATP by phosphate sensor based fluorescence assay | Homo sapiens | 2 | ALA4014351 | cell-based format | Scientific Literature | |
5. | ALA4017288 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 cells using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 12 | ALA4014351 | cell-based format | Scientific Literature | |
6. | ALA4017293 | B | Binding affinity to full length recombinant human N-terminal His-tagged BRR2 expressed in baculovirus infected Sf9 cells assessed as change in melting temperature at 100 uM by SYPRO orange dye based thermal shift assay | Homo sapiens | 2 | ALA4014351 | cell-based format | Scientific Literature | |
7. | ALA4017294 | B | Binding affinity to full length recombinant human N-terminal His-tagged BRR2 expressed in baculovirus infected Sf9 cells assessed as change in melting temperature at 33 uM by SYPRO orange dye based thermal shift assay | Homo sapiens | 2 | ALA4014351 | cell-based format | Scientific Literature | |
8. | ALA4017297 | B | Non-competitive inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 cells using varying levels of single stranded poly(U) RNA as substrate after 30 mins in presence of 25 uM ATP by ADP-Glo luminescence assay | Homo sapiens | 1 | ALA4014351 | cell-based format | Scientific Literature | |
9. | ALA4017298 | B | Non-competitive inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 cells using 2.5 ug/ml of single stranded poly(U) RNA as substrate after 30 mins in presence of varying levels of ATP by ADP-Glo luminescence assay | Homo sapiens | 1 | ALA4014351 | cell-based format | Scientific Literature | |
10. | ALA4017299 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 helicase activity expressed in baculovirus infected Sf9 cells assessed as reduction in RNA unwinding using 5'-[FITC]-UUCCCCUGCAUAAC-3'/5'-GUUAUGCAGGGGAACCAACGCAUAUCAGUGAGGAUU-3' RNA as substrate after 10 mins by PAGE analysis | Homo sapiens | 1 | ALA4014351 | cell-based format | Scientific Literature | |
11. | ALA4017300 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 helicase activity expressed in baculovirus infected Sf9 cells assessed as reduction in RNA unwinding at 100 uM using 5'-[FITC]-UUCCCCUGCAUAAC-3'/5'-GUUAUGCAGGGGAACCAACGCAUAUCAGUGAGGAUU-3' RNA as substrate after 10 mins by PAGE analysis | Homo sapiens | 1 | ALA4014351 | cell-based format | Scientific Literature | |
12. | ALA4044703 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 cells using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 30 | ALA4043122 | cell-based format | Scientific Literature | |
13. | ALA4044708 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 helicase activity expressed in baculovirus infected Sf9 cells using 5'-[FITC]-UUCCCCUGCAUAAC-3'/5'-GUUAUGCAGGGGAACCAACGCAUAUCAGUGAGGAUU-3' RNA as substrate after 10 mins in presence of ATP by PAGE analysis | Homo sapiens | 2 | ALA4043122 | cell-based format | Scientific Literature | |
14. | ALA4044709 | B | Inhibition of full length recombinant human N-terminal His-tagged BRR2 helicase activity expressed in baculovirus infected Sf9 cells using 5'-[FITC]-UUCCCCUGCAUAAC-3'/5'-GUUAUGCAGGGGAACCAACGCAUAUCAGUGAGGAUU-3' RNA as substrate at 100 uM after 10 mins in presence of ATP by PAGE analysis | Homo sapiens | 2 | ALA4043122 | cell-based format | Scientific Literature | |
15. | ALA4051072 | B | Inhibition of human recombinant full-length His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 insect cells using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 1 | ALA4049423 | cell-based format | Scientific Literature | |
16. | ALA4118649 | B | Binding affinity to SNRNP200 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | ALA4118021 | cell-based format | Scientific Literature | |
17. | ALA4140802 | B | Inhibition of human recombinant N-terminal full length His-tagged BRR2 RNA dependent ATPase activity expressed in baculovirus infected Sf9 insect cells using single stranded poly(U) RNA as substrate after 30 mins by ADP-Glo luminescence assay | Homo sapiens | 3 | ALA4138137 | cell-based format | Scientific Literature |