# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4030880 | B | Inhibition of UbcH5b (unknown origin) at 2.5 to 10 uM preincubated for 15 mins followed by E1, Ub and ATP addition measured after 40 mins by Western blot analysis | Homo sapiens | 1 | ALA4028851 | single protein format | Scientific Literature | |
2. | ALA4231160 | B | Inhibition of E2 (unknown origin) assessed as reduction in biotin-labelled ubiquitin-E2 conjugate formation at 10 to 1000 uM pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 60 mins in presence of E1 by Western blot analysis | Homo sapiens | 2 | ALA4229347 | single protein format | Scientific Literature | |
3. | ALA4231161 | B | Inhibition of E2 (unknown origin) assessed as reduction in biotin-labelled ubiquitin-E2 conjugate formation pretreated for 30 mins followed by biotin-labelled ubiquitin addition and measured after 60 mins in presence of E1 by Western blot analysis | Homo sapiens | 1 | ALA4229347 | single protein format | Scientific Literature |