# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4056544 | B | Inhibition of human CDK12/CyclinK at 1 uM by enzymatic radiometric assay | Homo sapiens | 2 | ALA4052710 | protein complex format | Scientific Literature | |
2. | ALA4151495 | B | Inhibition of N-terminal FLAG-tagged human full-length CDK12 (1 to 1490 residues)/N-terminal His-tagged CycK (1 to 580 residues) expressed in Sf9 cells assessed as reduction in ATP-dependent ULight-4E-BP1 (Thr37/Thr46) substrate peptide phosphorylation pre-incubated for 60 mins by LANCE Ultra assay | Homo sapiens | 30 | ALA4145674 | cell-based format | Scientific Literature | |
3. | ALA4190900 | B | Inhibition of recombinant full-length human CDK12/Cyclin K at 1 uM using RSRSRSRSRSRSRSR as substrate after 120 mins in presence of [gamma-33P]ATP by scintillation counting method | Homo sapiens | 1 | ALA4190187 | protein complex format | Scientific Literature | |
4. | ALA4314757 | B | Inhibition of recombinant full length human CDK12/cyclinK assessed as residual activity at 10 uM using RSRSRSRSRSRSRSR as substrate measured after 120 mins in presence of [gamma33P]ATP by scintillation counting method | Homo sapiens | 1 | ALA4311992 | protein complex format | Scientific Literature | |
5. | ALA4335008 | B | Inhibition of recombinant full length human CDK12/cyclinK assessed as residual activity at 1 uM using RSRSRSRSRSRSRSR as substrate incubated for 120 mins in presence of [gamma-33ATP] by scintillation counting based radiometry assay relative to control | Homo sapiens | 2 | ALA4334438 | protein complex format | Scientific Literature | |
6. | ALA4336836 | B | Inhibition of recombinant full length human CDK12/cyclinK assessed as residual activity at 10 uM using RSRSRSRSRSRSR as substrate incubated for 40 mins in presence of [gamma-33ATP] by scintillation counting based radiometry assay relative to control | Homo sapiens | 1 | ALA4334471 | protein complex format | Scientific Literature | |
7. | ALA4351791 | B | Inhibition of recombinant full length human CDK12/cyclinK at 10 uM using RSRSRSRSRSRSRSR as substrate measured after 120 mins in presence of [gamma33P]ATP by scintillation counting method | Homo sapiens | 1 | ALA4350961 | protein complex format | Scientific Literature | |
8. | ALA4377707 | B | Inhibition of human full length recombinant CDK12/cyclinK at 1 uM using RSRSRSRSRSRSRSR as substrate incubated for 120 mins in presence of [gamma-33P]ATP at by radiometric assay relative to control | Homo sapiens | 1 | ALA4376814 | protein complex format | Scientific Literature | |
9. | ALA4378936 | B | Inhibition of full-length recombinant human CDK12/cyclinK at 10 uM using RSRSRSRSRSRSRSR as substrate after 40 mins by in presence of [gamma33P]ATP by scintillation counting method relative to control | Homo sapiens | 1 | ALA4376829 | protein complex format | Scientific Literature | |
10. | ALA4379178 | B | Inhibition of recombinant full length human CDK12/cyclinK using RSRSRSRSRSRSR as substrate incubated for 120 mins in presence of [gamma-33ATP] by radiometric scintillation counting analysis | Homo sapiens | 1 | ALA4376834 | protein complex format | Scientific Literature | |
11. | ALA4408784 | B | Competitive irreversible inhibition of CDK12/cyclinK (unknown origin) in presence of 1 mM ATP | Homo sapiens | 1 | ALA4406837 | protein complex format | Scientific Literature | |
12. | ALA4408794 | B | Competitive irreversible inhibition of CDK12/cyclinK (unknown origin) in presence of Km ATP | Homo sapiens | 5 | ALA4406837 | protein complex format | Scientific Literature | |
13. | ALA4612776 | B | Inhibition of human CDK12/cyclin K using Pol2-CTD as substrate by [gamma-33P]ATP-based radioisotope filter binding assay | Homo sapiens | 1 | ALA4610118 | protein complex format | Scientific Literature | |
14. | ALA4678893 | B | Inhibition of human recombinant N-terminal GST-TEV tagged CDK12 (696 to 1082 residues)/full length human CyclinK expressed in baculovirus expression system using FITC-X-GSRTPMY-NH2 as substrate preincubated for 10 mins followed by MgCl2 addition and measured after 240 mins in presence of 5 mM ATP by mobility shift assay | Homo sapiens | 3 | ALA4673352 | assay format | Scientific Literature | |
15. | ALA4738623 | B | Inhibition of CDK12/cyclin K (unknown origin) using FAM-labeled peptide and ATP as substrate preincubated for 10 mins followed by substrate addition by mobility shift assay | Homo sapiens | 2 | ALA4732230 | protein complex format | Scientific Literature | |
16. | ALA4742485 | B | Inhibition of CDK12 (686 to 1082 residues) /Cyclin K (unknown origin) using 5-FAM-CDK9tide peptide substrate incubated for 3 hrs | Homo sapiens | 277 | ALA4742388 | protein complex format | Patent Bioactivity Data | |
17. | ALA4742612 | B | Inhibition of CDK12/cyclinK (unknown origin) using RBER-IRStide as substrate and in presence of ATP preincubated for 30 mins followed by addition of substrate measured after 1 hr by ADP-Glo reagent assay | Homo sapiens | 182 | ALA4742398 | protein complex format | Patent Bioactivity Data | |
18. | ALA4742634 | B | Inhibition of CDK12/cyclin K (unknown origin) assessed as incoporation of [33]Pi using RBER-IRStide as substrate and in presence of [gamma-33P]-ATP measured after 60 mins by microplate scintillation counter | Homo sapiens | 94 | ALA4742402 | protein complex format | Patent Bioactivity Data | |
19. | ALA4742635 | B | Inhibition of CDK12/cyclin K (unknown origin) assessed as incoporation of [33]Pi using RBER-IRStide as substrate and in presence of [gamma-33P]-ATP measured after 60 mins under thiol free condition by microplate scintillation counter | Homo sapiens | 14 | ALA4742402 | protein complex format | Patent Bioactivity Data | |
20. | ALA4775150 | B | Inhibition of human CDK12/human cyclin K expressed in baculovirus-infected insect cells using pol2 CTD-peptide substrate and [gammaP]ATP by scintillation counting method | Homo sapiens | 1 | ALA4765462 | cell-based format | Scientific Literature |