# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4198705 | B | Inhibition of Middle East respiratory syndrome-related coronavirus 3CL protease using Dabcyl-KTSAVLQ/SGFRKME-Edans as substrate incubated for 30 mins followed by substrate addition measured after 1 hr by FRET assay | Middle East respiratory syndrome-related coronavirus | 12 | single protein format | Scientific Literature | ||
2. | ALA4393309 | B | Inhibition of MERS-CoV papain-like protease using Z-Arg-Leu-Arg-Gly-Gly-AMC as substrate preincubated for 5 mins followed by substrate addition and measured for 10 mins by fluorescence assay | Middle East respiratory syndrome-related coronavirus | 11 | single protein format | Scientific Literature | ||
3. | ALA4393310 | B | Inhibition of MERS-CoV papain-like protease at 50 uM using Z-Arg-Leu-Arg-Gly-Gly-AMC as substrate preincubated for 5 mins followed by substrate addition and measured for 10 mins by fluorescence assay relative to control | Middle East respiratory syndrome-related coronavirus | 2 | single protein format | Scientific Literature | ||
4. | ALA4393311 | B | Binding affinity to MERS-CoV papain-like protease assessed as association rate constant by SPR analysis | Middle East respiratory syndrome-related coronavirus | 2 | single protein format | Scientific Literature | ||
5. | ALA4393312 | B | Binding affinity to MERS-CoV papain-like protease assessed as dissociation rate constant by SPR analysis | Middle East respiratory syndrome-related coronavirus | 2 | single protein format | Scientific Literature | ||
6. | ALA4393313 | B | Binding affinity to MERS-CoV papain-like protease by SPR analysis | Middle East respiratory syndrome-related coronavirus | 8 | single protein format | Scientific Literature | ||
7. | ALA4393314 | B | Competitive-inhibition of MERS-CoV papain-like protease using varying levels of Z-Arg-Leu-Arg-Gly-Gly-AMC as substrate by Dixon-plot analysis | Middle East respiratory syndrome-related coronavirus | 2 | single protein format | Scientific Literature | ||
8. | ALA4393315 | B | Binding affinity to MERS-CoV papain-like protease in presence of Z-Arg-Leu-Arg-Gly-Gly-AMC by SPR analysis | Middle East respiratory syndrome-related coronavirus | 1 | single protein format | Scientific Literature | ||
9. | ALA4393316 | B | Ratio of Kd for MERS-CoV papain-like protease in presence of Z-Arg-Leu-Arg-Gly-Gly-AMC to Kd for MERS-CoV papain-like protease in absence of Z-Arg-Leu-Arg-Gly-Gly-AMC | Middle East respiratory syndrome-related coronavirus | 1 | single protein format | Scientific Literature | ||
10. | ALA4393317 | B | Reversible inhibition of MERS-CoV papain-like protease assessed as recovery of enzyme activity at 125 times IC50 using Z-Arg-Leu-Arg-Gly-Gly-AMC as substrate incubated for 1 hr followed by 2-fold dilution and measured by fluorescence assay | Middle East respiratory syndrome-related coronavirus | 1 | single protein format | Scientific Literature | ||
11. | ALA4393318 | B | Binding affinity to 15N-labelled His-tagged Apo-MRES-CoV papain-like protease by TROSY [1H-15N] spectroscopy | Middle East respiratory syndrome-related coronavirus | 1 | single protein format | Scientific Literature | ||
12. | ALA4495584 | F | MERS_3CL Pro protease inhibition IC50 by FRET kind of response from peptide substrate | Middle East respiratory syndrome-related coronavirus | 80 | assay format | SARS-CoV-2 Screening Data 2020-21 | ||
13. | ALA4495585 | F | MERS 3CL-Pro protease inhibition percentage at 10 µM by FRET kind of response from peptide substrate | Middle East respiratory syndrome-related coronavirus | 70 | assay format | SARS-CoV-2 Screening Data 2020-21 | ||
14. | ALA5044401 | B | Inhibition of MERS PLpro using LRGG-ACC as substrate upto 1000 uM preincubated for 60 mins followed by substrate addition and measured for 30 mins by enzymatic assay | Middle East respiratory syndrome-related coronavirus | 4 | single protein format | Scientific Literature | ||
15. | ALA5231856 | B | Inhibition of recombinant MERS-CoV PL protease using Ub-AFC incubated for 3 mins by fluorescence based analysis | Middle East respiratory syndrome-related coronavirus | 1 | single protein format | Scientific Literature |