# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4144828 | B | Displacement of IK-698 from Escherichia coli LeuRS by isothermal titration calorimetric calorimetry | Escherichia coli | 9 | single protein format | Scientific Literature | ||
2. | ALA4144829 | B | Binding affinity to Escherichia coli LeuRS by isothermal titration calorimetric calorimetry | Escherichia coli | 1 | single protein format | Scientific Literature | ||
3. | ALA4144830 | B | Inhibition of Escherichia coli LeuRS expressed in Escherichia coli M15 cells assessed as reduction in ATP consumption | Escherichia coli | 10 | cell-based format | Scientific Literature | ||
4. | ALA4197602 | B | Inhibition of Leucyl-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled leucine addition measured after 5 mins by liquid scintillation counting method relative to control | Escherichia coli K-12 | 2 | single protein format | Scientific Literature | ||
5. | ALA4197608 | B | Inhibition of Escherichia coli BL21(DE3) Leucyl-tRNA synthetase assessed as reduction in tRNA aminoacylation preincubated for 10 mins with Escherichia coli tRNA and 14C-labeled leucine followed by ATP addition by liquid scintillation counting method | Escherichia coli BL21(DE3) | 2 | single protein format | Scientific Literature | ||
6. | ALA4374110 | B | Binding affinity to Escherichia coli LeuRS | Escherichia coli | 1 | single protein format | Scientific Literature | ||
7. | ALA4394777 | B | Inhibition of leuRS in Escherichia coli NCIB 8743 S30 extract assessed as aminoacylation at 250 nM incubated for 5 mins in presence of 14C-labeled leucine by liquid scintillation counting method relative to control | Escherichia coli | 4 | single protein format | Scientific Literature | ||
8. | ALA4394780 | B | Inhibition of purified Escherichia coli BL21 (DE3) leuRS incubated for 10 mins in presence of 14C-labeled leucine by radiolabel transfer assay | Escherichia coli BL21(DE3) | 3 | single protein format | Scientific Literature | ||
9. | ALA4394787 | B | Inhibition of leuRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing RimI incubated for 5 to 60 mins in presence of 14C-labeled leucine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 9 | single protein format | Scientific Literature | ||
10. | ALA4394790 | B | Inhibition of leuRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing SUMO incubated for 5 to 60 mins in presence of 14C-labeled leucine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 9 | single protein format | Scientific Literature | ||
11. | ALA4394793 | B | Inhibition of leuRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing RimL incubated for 5 to 60 mins in presence of 14C-labeled leucine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 6 | single protein format | Scientific Literature | ||
12. | ALA4394796 | B | Inhibition of leuRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing YhhY incubated for 5 mins in presence of 14C-labeled leucine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 3 | single protein format | Scientific Literature | ||
13. | ALA4394797 | B | Inhibition of leuRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing YhhY incubated for 60 mins in presence of 14C-labeled leucine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 3 | single protein format | Scientific Literature | ||
14. | ALA4819635 | B | Inhibition of recombinant Escherichia coli LeuRS assessed as inhibition of 14C-radiolabelled leucine transfer to tRNA leucine preincubated for 10 mins followed by addition of ATP and measured after 4 mins by Scintillation counting method | Escherichia coli | 12 | single protein format | Scientific Literature |