# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4197603 | B | Inhibition of Tyrosyl-tRNA synthetase in Escherichia coli K-12 BW28357 S30 extract assessed as reduction in tRNA aminoacylation at 2.5 uM preincubated for 5 mins followed by Escherichia coli tRNA and 14C-labeled tyrosine addition measured after 5 mins by liquid scintillation counting method relative to control | Escherichia coli K-12 | 2 | single protein format | Scientific Literature | ||
2. | ALA4197609 | B | Inhibition of Escherichia coli BL21(DE3) Tyrosyl-tRNA synthetase assessed as reduction in tRNA aminoacylation preincubated for 10 mins with Escherichia coli tRNA and 14C-labeled tyrosine followed by ATP addition by liquid scintillation counting method | Escherichia coli BL21(DE3) | 2 | single protein format | Scientific Literature | ||
3. | ALA4394776 | B | Inhibition of purified Escherichia coli BL21 (DE3) tyrRS incubated for 10 mins in presence of 14C-labeled tyrosine by radiolabel transfer assay | Escherichia coli BL21(DE3) | 3 | single protein format | Scientific Literature | ||
4. | ALA4394778 | B | Inhibition of tyrRS in Escherichia coli NCIB 8743 S30 extract assessed as aminoacylation at 250 nM incubated for 5 mins in presence of 14C-labeled tyrosine by liquid scintillation counting method relative to control | Escherichia coli | 4 | single protein format | Scientific Literature | ||
5. | ALA4394788 | B | Inhibition of tyrRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing RimI incubated for 5 to 60 mins in presence of 14C-labeled tyrosine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 9 | single protein format | Scientific Literature | ||
6. | ALA4394791 | B | Inhibition of tyrRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing SUMO incubated for 5 to 60 mins in presence of 14C-labeled tyrosine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 9 | single protein format | Scientific Literature | ||
7. | ALA4394798 | B | Inhibition of tyrRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing YhhY incubated for 5 to 60 mins in presence of 14C-labeled tyrosine by liquid scintillation counting method | Escherichia coli BL21(DE3) | 3 | single protein format | Scientific Literature | ||
8. | ALA4394799 | B | Inhibition of tyrRS in Escherichia coli BL21 (DE3) pLysS S30 extract over expressing RimL incubated for 5 to 60 mins in presence of 14C-labeled tyrosine by liquid scintillation counting method relative to control | Escherichia coli BL21(DE3) | 2 | single protein format | Scientific Literature | ||
9. | ALA4625541 | B | Inhibition of Escherichia coli BL21(DE3) TyrRS overexpressed in Escherichia coli Rosetta2(DE3) assessed as reduction in tRNA aminoacylation at 5 uM preincubated with enzyme and substrate for 10 mins prior to ATP addition using 14C-labeled tyrosine and tRNA as substrate by liquid scintillation counting method | Escherichia coli BL21(DE3) | 3 | assay format | Scientific Literature |