#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4202305	Binding	Inhibition of recombinant Trypanosoma brucei brucei alternative oxidase using ubiquinol-1 as substrate incubated for 2 mins followed by substrate addition by spectrophotometric method	Trypanosoma brucei brucei	35	ALA4196235	single protein format	Scientific Literature
2.	ALA4202316	Binding	Binding affinity to recombinant Trypanosoma brucei brucei 427 alternative oxidase expressed in Escherichia coli BL21 cells using ubiquinol-1 as substrate by spectrophotometric method	Trypanosoma brucei brucei	1	ALA4196235	cell-based format	Scientific Literature
3.	ALA4252096	Binding	Inhibition of Trypanosoma brucei alternative oxidase expressed in Escherichia coli FN102 using ubiquinol-1 as substrate preincubated for 2 mins followed by substrate addition by spectrophotometric method	Trypanosoma brucei	1	ALA4251554	assay format	Scientific Literature
4.	ALA4252097	Binding	Inhibition of recombinant His-tagged and SUMO-tagged Trypanosoma alternative oxidase expressed in Escherichia coli Rosetta2 using 1-ubiquinol as substrate measured over 6 mins by spectrophotometric method	Trypanosoma	17	ALA4251554	assay format	Scientific Literature
5.	ALA4252099	Binding	Mixed type non-competitive inhibition of recombinant His-tagged and SUMO-tagged Trypanosoma alternative oxidase expressed in Escherichia coli Rosetta2 at 1 to 10 nM using varying levels of 1-ubiquinol as substrate measured over 6 mins by spectrophotometric metho	Trypanosoma	1	ALA4251554	assay format	Scientific Literature
6.	ALA4838018	Binding	Inhibition of recombinant Trypanosoma brucei brucei alternative oxidase using ubiquinol as substrate preincubated for 2 mins followed by substrate addition by UV-Vis spectrophotometry	Trypanosoma brucei brucei	10	ALA4837204	single protein format	Scientific Literature