# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4270013 | B | Inhibition of recombinant Staphylococcus aureus SaeS autophosphorylation expressed in Escherichia coli BL21(DE3) preincubated for 15 mins followed by [gamma-32P]-ATP addition measured after 15 mins by coomassie-brilliant blue staining based SDS PAGE//autoradiographic analysis | Staphylococcus aureus | 2 | ALA4266038 | assay format | Scientific Literature | |
2. | ALA4270017 | B | Binding affinity to Staphylococcus aureus SaeS kinase domain after 15 mins by fluorescence spectrophotometry | Staphylococcus aureus | 2 | ALA4266038 | single protein format | Scientific Literature | |
3. | ALA4270018 | B | Binding affinity to Staphylococcus aureus MBP-fused SaeS by STD-NMR spectrophotometry | Staphylococcus aureus | 1 | ALA4266038 | single protein format | Scientific Literature | |
4. | ALA4270022 | B | Inhibition of phosphotransfer reaction from recombinant Staphylococcus aureus phosphorylated His6-tagged SaeS kinase domain to Staphylococcus aureus SaeR expressed in Escherichia coli BL21(DE3) at 0.2 mM after 60 mins by coomassie-brilliant blue staining based SDS PAGE/autoradiographic analysis | Staphylococcus aureus | 1 | ALA4266038 | assay format | Scientific Literature | |
5. | ALA4270023 | B | Inhibition of phosphotransfer reaction from recombinant Staphylococcus aureus phosphorylated His6-tagged SaeS kinase domain to Staphylococcus aureus SaeR expressed in Escherichia coli BL21(DE3) at 0.2 to 1 mM after 60 mins by coomassie-brilliant blue staining based SDS PAGE/autoradiographic analysis | Staphylococcus aureus | 1 | ALA4266038 | assay format | Scientific Literature | |
6. | ALA4270024 | B | Binding affinity to Staphylococcus aureus SaeS assessed as T2-rerelaxation time of compound peak at 1 mM by 1D CPMG method | Staphylococcus aureus | 1 | ALA4266038 | single protein format | Scientific Literature | |
7. | ALA4270025 | B | Binding affinity to Staphylococcus aureus SaeS kinase domain at 1 mM by 1D-NMR analysis | Staphylococcus aureus | 1 | ALA4266038 | single protein format | Scientific Literature |