# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4265620 | B | Inhibition of N-terminal His6-tagged Staphylococcus aureus PanK2 expressed in Escherichia coli BL21(DE3) cells at 200 uM using pantothenate as substrate in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay | Staphylococcus aureus | 2 | cell-based format | Scientific Literature | ||
2. | ALA4265621 | B | Inhibition of N-terminal His6-tagged Staphylococcus aureus PanK2 expressed in Escherichia coli BL21(DE3) cells at >= 100 uM using pantothenate as substrate in presence of ATP by pyruvate kinase/lactate dehydrogenase enzyme coupled assay | Staphylococcus aureus | 14 | cell-based format | Scientific Literature | ||
3. | ALA4265625 | B | Substrate activity at N-terminal His6-tagged Staphylococcus aureus PanK2 expressed in Escherichia coli BL21(DE3) cells assessed as Km in presence of ATP/NADH by pyruvate kinase/lactate dehydrogenase enzyme coupled assay | Staphylococcus aureus | 10 | cell-based format | Scientific Literature | ||
4. | ALA4265626 | B | Substrate activity at N-terminal His6-tagged Staphylococcus aureus PanK2 expressed in Escherichia coli BL21(DE3) cells assessed as Kcat in presence of ATP/NADH by pyruvate kinase/lactate dehydrogenase enzyme coupled assay | Staphylococcus aureus | 10 | cell-based format | Scientific Literature | ||
5. | ALA4265627 | B | Substrate activity at N-terminal His6-tagged Staphylococcus aureus PanK2 expressed in Escherichia coli BL21(DE3) cells assessed as ratio of Kcat to Km in presence of ATP/NADH by pyruvate kinase/lactate dehydrogenase enzyme coupled assay | Staphylococcus aureus | 12 | cell-based format | Scientific Literature | ||
6. | ALA4265637 | B | Substrate activity at N-terminal His6-tagged Staphylococcus aureus PanK2 expressed in Escherichia coli BL21(DE3) cells in presence of ATP/NADH by pyruvate kinase/lactate dehydrogenase enzyme coupled assay | Staphylococcus aureus | 1 | cell-based format | Scientific Literature |