# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4265026 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of CoCl2 by fluorescence assay | Streptococcus pneumoniae | 9 | cell-based format | Scientific Literature | ||
2. | ALA4265027 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid at 5 mM using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of CoCl2 by fluorescence assay | Streptococcus pneumoniae | 2 | cell-based format | Scientific Literature | ||
3. | ALA4265028 | B | Inhibition of His6-tagged Streptococcus pneumoniae Pgda C-terminal de-N-acetylase domain (232 to 431 residues) expressed in Escherichia coli BL21 (DE3) cells transformed with pET28b-SpPgdA232-431 using chitotriose as substrate measured at 75 or 100 sec intervals up to 5 mins in presence of CoCl2 by fluorescence assay | Streptococcus pneumoniae | 2 | cell-based format | Scientific Literature | ||
4. | ALA4265030 | B | Substrate activity at Streptococcus pneumoniae Pgda at 5 mM in presence of CoCl2 or ZnCl2 by fluorescamine assay deacetylase assay | Streptococcus pneumoniae | 4 | single protein format | Scientific Literature | ||
5. | ALA4265031 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid at 200 to 1200 uM using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of 3 uM ZnCl2 by fluorescence assay | Streptococcus pneumoniae | 1 | cell-based format | Scientific Literature | ||
6. | ALA4265032 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid at 200 to 1200 uM using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of 6 uM ZnCl2 by fluorescence assay | Streptococcus pneumoniae | 1 | cell-based format | Scientific Literature | ||
7. | ALA4265033 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid at 200 to 1200 uM using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of 200 uM ZnCl2 by fluorescence assay | Streptococcus pneumoniae | 1 | cell-based format | Scientific Literature | ||
8. | ALA4265034 | B | Partial mixed inhibition of Streptococcus pneumoniae apo-Pgda using N,N',N\"-triacetyl chitotriose as substrate in presence of ZnCl2 by fluorescamine assay deacetylase assay based Dixon plot analysis | Streptococcus pneumoniae | 1 | single protein format | Scientific Literature | ||
9. | ALA4265038 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid using compound pre-incubated with 1Eq DTT and using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of CoCl2 by fluorescence assay | Streptococcus pneumoniae | 3 | cell-based format | Scientific Literature | ||
10. | ALA4265039 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid using compound pre-incubated with 1Eq DTT and using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of ZnCl2 by fluorescence assay | Streptococcus pneumoniae | 3 | cell-based format | Scientific Literature | ||
11. | ALA4265040 | B | Inhibition of Streptococcus pneumoniae Pgda expressed in Escherichia coli BL21 (DE3) cells transformed with pET28bSpPgdA232-431 plasmid at 10 mM using acetoxymethyl-4-methylumbelliferone as substrate measured at 5 min intervals over 60 mins in presence of CoCl2 by fluorescence assay | Streptococcus pneumoniae | 1 | cell-based format | Scientific Literature | ||
12. | ALA4265042 | B | Substrate activity at Streptococcus pneumoniae Pgda at 1 mM in presence of CoCl2 or ZnCl2 by fluorescamine assay deacetylase assay | Streptococcus pneumoniae | 3 | single protein format | Scientific Literature |