# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4186217 | B | Competitive inhibition of Caulobacter vibrioides full length wild type Cell cycle histidine kinase CckA deltaTM expressed in Escherichia coli BL21-AI assessed as enzyme Km in presence of varying levels of ATP measured every 60 secs for 2 hrs by Lineweaver-Burk plot analysis | Caulobacter vibrioides | 1 | ALA4184156 | assay format | Scientific Literature | |
2. | ALA4186218 | B | Competitive inhibition of Caulobacter vibrioides full length wild type cell cycle histidine kinase CckA deltaTM Escherichia coli BL21-AI in presence of varying levels of ATP measured every 60 secs for 2 hrs by Lineweaver-Burk plot analysis | Caulobacter vibrioides | 1 | ALA4184156 | single protein format | Scientific Literature | |
3. | ALA4186231 | B | Binding affinity to recombinant Caulobacter vibrioides cell cycle histidine kinase CckA delta 3 mutant DHp domain (190 to 562 residues) expressed in Escherichia coli assessed as change in metlting temperature at 30 uM after 2 mins by SYPRO orange dye based DSF assay | Caulobacter vibrioides | 16 | ALA4184156 | assay format | Scientific Literature | |
4. | ALA4186232 | B | Inhibition of recombinant Caulobacter vibrioides cell cycle histidine kinase CckA deltaTM mutant DHp domain (70 to 691 residues) expressed in Escherichia coli in presence of varying levels of ATP measured every 60 secs for 2 hrs by PK/LDH enzyme coupled assay | Caulobacter vibrioides | 16 | ALA4184156 | assay format | Scientific Literature | |
5. | ALA4186233 | B | Inhibition of recombinant Caulobacter vibrioides cell cycle histidine kinase CckA deltaTM mutant DHp domain (70 to 691 residues) expressed in Escherichia coli at 250 uM in presence of varying levels of ATP measured every 60 secs for 2 hrs by PK/LDH enzyme coupled assay relative to control | Caulobacter vibrioides | 3 | ALA4184156 | assay format | Scientific Literature |