# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4188598 | B | Inhibition of mouse NNMT at 30 uM preincubated for 30 mins followed by nicotinamide substrate and SAM addition measured after 60 mins by fluorescence assay | Mus musculus | 2 | ALA4184280 | single protein format | Scientific Literature | |
2. | ALA4188600 | B | Inhibition of NNMT in mouse 3T3L1 cells assessed as reduction in MNA production after 24 hrs by LC-MS/MS analysis | Mus musculus | 2 | ALA4184280 | cell-based format | Scientific Literature | |
3. | ALA4714714 | B | Inhibition of mouse NNMT using nicotinamide as substrate incubated for 2 hrs in presence of S-5'adenosyl-L-methionine by RapidFire Mass spectroscopy | Mus musculus | 1 | ALA4706725 | single protein format | Scientific Literature | |
4. | ALA4810080 | B | Inhibition of mouse recombinant NNMT using nicotinamide and SAM as substrate assessed as reduction in 1-methyl-nicotinamide formation incubated for 22 to 24 hrs by RapidFire Mass spectroscopy | Mus musculus | 27 | ALA4808207 | single protein format | Scientific Literature | |
5. | ALA4810085 | B | Inhibition of mouse recombinant NNMT using nicotinamide and SAM as substrate assessed as reduction in 1-methyl-nicotinamide formation incubated for 2 hrs by RapidFire Mass spectroscopy | Mus musculus | 2 | ALA4808207 | single protein format | Scientific Literature | |
6. | ALA5223198 | B | Inhibition of mouse NNMT preincubated for 30 mins followed by substrate, cofactor addition and measured after 60 mins using nicotinamide as substrate and SAM as cofactor by fluorescence enzymatic assay | Mus musculus | 1 | ALA5223061 | single protein format | Scientific Literature | |
7. | ALA5223201 | B | Inhibition of endogenous mouse NNMT overexpressed in murine 3T3-L1 cells assessed incubated for 24 hrs by LC-MS/MS analysis | Mus musculus | 1 | ALA5223061 | cell-based format | Scientific Literature |