# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA979114 | B | Inhibition of basal activity of adenylyl cyclase 2 expressed in HEK293 cells at 100 uM | 2 | cell-based format | Scientific Literature | |||
2. | ALA979117 | B | Stimulation of basal activity of adenylyl cyclase 2 expressed in HEK293 cells at 100 uM in presence of forskolin | 1 | cell-based format | Scientific Literature | |||
3. | ALA4384524 | B | Inhibition of human AC2 expressed in HEK293 cells assessed as PMA-stimulated cAMP accumulation at 30 uM preincubated for 30 mins followed by PMA stimulation and measured after 1 hr by fluorescence assay relative to control | Homo sapiens | 7 | cell-based format | Scientific Literature | ||
4. | ALA4603679 | B | Agonist activity at recombinant human AC2 expressed in human HEK293 cells assessed as increase in cAMP level at 50 uM by LANCE Ultra cAMP Detection kit method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
5. | ALA4685438 | B | Agonist activity at recombinant human AC2 expressed in HEK293 cells assessed as increase in cAMP level at 50 uM measured after 30 mins by LANCE Ultra cAMP Detection kit method | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
6. | ALA4685439 | B | Agonist activity at recombinant human AC2 expressed in HEK293 cells assessed as increase in cAMP level measured after 30 mins by LANCE Ultra cAMP Detection kit method | Homo sapiens | 41 | cell-based format | Scientific Literature | ||
7. | ALA4685443 | B | Agonist activity at recombinant human AC2 expressed in HEK293 cells assessed as increase in cAMP level by measuring cAMP level at 50 uM measured after 30 mins by LANCE Ultra cAMP Detection kit method relative to control | Homo sapiens | 59 | cell-based format | Scientific Literature | ||
8. | ALA4685444 | B | Potency index, ratio of forskolin EC50 to test compound EC50 for agonist activity at recombinant human AC2 expressed in HEK293 cells assessed as increase in cAMP level | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
9. | ALA4685445 | B | Agonist activity at recombinant human AC2 expressed in HEK293 cells assessed as reduction in IL6 level by measuring IL6 level at 50 uM measured after 24 hrs by ELISA relative to control | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
10. | ALA4824401 | B | Inhibition of human AC2 expressed in HEK293 cells assessed as remaining activity by measuring cAMP level at 30 uM relative to control | Homo sapiens | 24 | cell-based format | Scientific Literature | ||
11. | ALA5161382 | B | Potentiation of PMA-stimulted human AC2 activity in AC3 and AC6-knockout HEK293 cells PMA stimulated cAMP accumulation at 10 uM incubated for 30 mins followed by PMA stimulation in presence of IBMX for 1 hr by HTRF assay relative to control | Homo sapiens | 5 | cell-based format | Scientific Literature | ||
12. | ALA5218110 | B | Inhibition of human ADCY2 assessed assessed as cAMP accumulation preincubated for 15 mins followed by substrate addition using alpha-32P labelled ATP as substrate by sequential chromatographic analysis | Homo sapiens | 1 | single protein format | Scientific Literature |