Assay

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Assay

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#	Aladdin ID	Assay Type	Description	Organism	Compounds	BAO Format	Source
1.	ALA4118972	B	Binding affinity to SHMT2 in human NCI-H358 cells at 1 uM by mass spectrometry based pull down assay	Homo sapiens	1	cell-based format	Scientific Literature
2.	ALA4418847	B	Inhibition of His-tagged human recombinant SHMT2 expressed in Escherichia coli BLR(DE3) assessed as reduction in NADPH level using L-serine, THF and NADP+ at 6.5 or 26.5 uM incubated for 5 mins by SHMT2-MTHFD coupled reaction based fluorescence assay relative to control	Homo sapiens	46	assay format	Patent Bioactivity Data
3.	ALA4418848	B	Inhibition of His-tagged human recombinant SHMT2 expressed in Escherichia coli BLR(DE3) assessed as reduction in NADPH level using L-serine, THF and NADP+ incubated for 5 mins by SHMT2-MTHFD coupled reaction based fluorescence assay	Homo sapiens	195	assay format	Patent Bioactivity Data