| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4202266 | B | Displacement of N-Flu-DOT1L from MBP-tagged AF9 (unknown origin) (487 to 568 residues) expressed in Escherichia coli BL21(DE3) measured after 3 hrs by fluorescence polarization assay | Homo sapiens | 28 | assay format | Scientific Literature | ||
| 2. | ALA4202268 | B | Binding affinity to MBP-tagged AF9 (unknown origin) (487 to 568 residues) expressed in Escherichia coli BL21(DE3) by Bio-layer interferometry | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 3. | ALA4202271 | B | Binding affinity to Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells measured after overnight incubation by pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA4202272 | B | Displacement of (S)-N-((S)-1-((S)-3-amino-1-((S)-3-cyclopentyl-1-((S)-2-((S)-1-(dimethylamino)-4-methyl-1-oxopentan-2-ylcarbamoyl)pyrrolidin-1-yl)-1-oxopropan-2-ylamino)-1-oxopropan-2-ylamino)-3-methyl-1-oxobutan-2-yl)-1-((S)-4-methyl-2-(6-(5-((3aR,4S,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)pentanoyl)pyrrolidine-2-carboxamide from Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells measured after overnight incubation by pull down assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4202273 | B | Displacement of (S)-N-((S)-1-((S)-3-amino-1-((S)-3-cyclopentyl-1-((S)-2-((S)-1-(dimethylamino)-4-methyl-1-oxopentan-2-ylcarbamoyl)pyrrolidin-1-yl)-1-oxopropan-2-ylamino)-1-oxopropan-2-ylamino)-3-methyl-1-oxobutan-2-yl)-1-((S)-4-methyl-2-(6-(5-((3aR,4S,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)pentanoyl)pyrrolidine-2-carboxamide from Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 100 nM measured after overnight incubation by pull down assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4202274 | B | Displacement of (S)-N-((S)-1-((S)-3-amino-1-((S)-3-cyclopentyl-1-((S)-2-((S)-1-(dimethylamino)-4-methyl-1-oxopentan-2-ylcarbamoyl)pyrrolidin-1-yl)-1-oxopropan-2-ylamino)-1-oxopropan-2-ylamino)-3-methyl-1-oxobutan-2-yl)-1-((S)-4-methyl-2-(6-(5-((3aR,4S,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)pentanoyl)pyrrolidine-2-carboxamide from Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 0.3 uM measured after overnight incubation by pull down assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4202275 | B | Displacement of (S)-N-((S)-1-((S)-3-amino-1-((S)-3-cyclopentyl-1-((S)-2-((S)-1-(dimethylamino)-4-methyl-1-oxopentan-2-ylcarbamoyl)pyrrolidin-1-yl)-1-oxopropan-2-ylamino)-1-oxopropan-2-ylamino)-3-methyl-1-oxobutan-2-yl)-1-((S)-4-methyl-2-(6-(5-((3aR,4S,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)pentanoyl)pyrrolidine-2-carboxamide from Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 1 uM measured after overnight incubation by pull down assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4202276 | B | Displacement of (S)-N-((S)-1-((S)-3-amino-1-((S)-3-cyclopentyl-1-((S)-2-((S)-1-(dimethylamino)-4-methyl-1-oxopentan-2-ylcarbamoyl)pyrrolidin-1-yl)-1-oxopropan-2-ylamino)-1-oxopropan-2-ylamino)-3-methyl-1-oxobutan-2-yl)-1-((S)-4-methyl-2-(6-(5-((3aR,4S,6aS)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)hexanamido)pentanoyl)pyrrolidine-2-carboxamide from Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 3 uM measured after overnight incubation by pull down assay relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4202277 | B | Inhibition of FLAG-tagged DOT1L binding to Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells measured after overnight incubation by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4202278 | B | Inhibition of FLAG-tagged DOT1L binding to Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 0.3 uM measured after overnight incubation by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4202279 | B | Inhibition of FLAG-tagged DOT1L binding to Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 1 uM measured after overnight incubation by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4202280 | B | Inhibition of FLAG-tagged DOT1L binding to Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 3 uM measured after overnight incubation by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4202281 | B | Inhibition of FLAG-tagged DOT1L binding to Myc-tagged AF9 CxxC domain (unknown origin) expressed in HEK293T cells at 10 uM measured after overnight incubation by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4810341 | B | Inhibition of human His-AF9 YEATS domain and biotinylated H3K9ac peptide interaction by AlphaScreen assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 15. | ALA4810346 | B | Inhibition of human His-AF9 YEATS domain and biotinylated H3K9ac peptide interaction at 20 uM by AlphaScreen assay | Homo sapiens | 4 | single protein format | Scientific Literature | ||
| 16. | ALA4810358 | B | Stabilization of AF9 in human HeLa cells assessed as inhibition of high temperature-induced AF9 protein degradation at 20 uM incubated for 5 hrs by cellular thermal shift assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 17. | ALA4837025 | B | Inhibition of AF9 YEATS domain (1 to 138 residues) (unknown origin) assessed as inhibition of probe-1 induced AF9 YEATS domain labelling incubated for 10 mins followed by UV-irradiation for 20 mins by Photo-cross linking based In-gel fluorescence scanning analysis | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 18. | ALA4837026 | B | Binding affinity to AF9 YEATS domain (1 to 138 residues) (unknown origin) by isothermal titration calorimetry | Homo sapiens | 2 | single protein format | Scientific Literature | ||
| 19. | ALA4837027 | B | Inhibition of sfGFP-fused AF9 (unknown origin) transfected in human U2OS cells assessed as inhibition of YEATS-dependent AF9-chromatin association by measuring fluorescence recovery rate at 20 uM by FRAP assay (Rvb = 1.91 s) | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 20. | ALA5069306 | B | Homogeneous Time Resolved Fluorescence (HTRF) assay. Domain start/stop: M1-D141 | Homo sapiens | 1 | single protein format | EUbOPEN Chemogenomic Library |
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