# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4202267 | Binding | Displacement of N-Flu-DOT1L from MBP-tagged ENL (unknown origin) (489 to 559 residues) expressed in Escherichia coli BL21(DE3) measured after 3 hrs by fluorescence polarization assay | Homo sapiens | 28 | ALA4196231 | assay format | Scientific Literature | |
2. | ALA4202269 | Binding | Binding affinity to MBP-tagged ENL (unknown origin) (489 to 559 residues) expressed in Escherichia coli BL21(DE3) by Bio-layer interferometry | Homo sapiens | 1 | ALA4196231 | assay format | Scientific Literature | |
3. | ALA4265379 | Binding | Binding affinity to recombinant C-terminal His6-tagged ENL YEATS domain (1 to 148 residues) (unknown origin) expressed in expressed Escherichia coli Rosetta assessed as change in melting temperature by thermal shift assay | Homo sapiens | 19 | ALA4261641 | assay format | Scientific Literature | |
4. | ALA4265380 | Binding | Binding affinity to recombinant C-terminal His6-tagged ENL YEATS domain (1 to 148 residues) (unknown origin) expressed in expressed Escherichia coli Rosetta by ITC assay | Homo sapiens | 5 | ALA4261641 | assay format | Scientific Literature | |
5. | ALA4411218 | Binding | Inhibition of human MLLT1 by alphascreen assay | Homo sapiens | 1 | ALA4406924 | single protein format | Scientific Literature | |
6. | ALA4411219 | Binding | Binding affinity to human MLLT1 by isothermal titration calorimetry | Homo sapiens | 1 | ALA4406924 | single protein format | Scientific Literature | |
7. | ALA4411220 | Binding | Binding affinity to C-terminal His6-tagged MLLT1 YEATS domain (1 to 148 residues) (unknown origin) expressed in Escherichia coli Rosetta by isothermal titration calorimetry | Homo sapiens | 6 | ALA4406924 | assay format | Scientific Literature | |
8. | ALA4411221 | Binding | Inhibition of C-terminal His6-tagged MLLT1 YEATS domain (1 to 148 residues) (unknown origin) expressed in Escherichia coli Rosetta by alphascreen assay | Homo sapiens | 6 | ALA4406924 | assay format | Scientific Literature | |
9. | ALA4411222 | Binding | Binding affinity to C-terminal His6-tagged MLLT1 YEATS domain (1 to 148 residues) (unknown origin) expressed in Escherichia coli Rosetta assessed as change in enthalpy by isothermal titration calorimetry | Homo sapiens | 6 | ALA4406924 | assay format | Scientific Literature | |
10. | ALA4411223 | Binding | Binding affinity to C-terminal His6-tagged MLLT1 YEATS domain (1 to 148 residues) (unknown origin) expressed in Escherichia coli Rosetta assessed as change in entropy by isothermal titration calorimetry | Homo sapiens | 6 | ALA4406924 | assay format | Scientific Literature | |
11. | ALA4411224 | Binding | Binding affinity to C-terminal His6-tagged MLLT1 YEATS domain (1 to 148 residues) (unknown origin) expressed in Escherichia coli Rosetta assessed as change in Gibb's free energy by isothermal titration calorimetry | Homo sapiens | 6 | ALA4406924 | assay format | Scientific Literature | |
12. | ALA4810338 | Binding | Inhibition of human His-ENL YEATS domain and biotinylated H3K9ac peptide interaction by AlphaScreen assay | Homo sapiens | 28 | ALA4808215 | single protein format | Scientific Literature | |
13. | ALA4810339 | Binding | Binding affinity to human His-ENL YEATS domain assessed as dissociation constant by SPR analysis | Homo sapiens | 2 | ALA4808215 | single protein format | Scientific Literature | |
14. | ALA4810344 | Binding | Inhibition of human His-ENL YEATS domain and biotinylated H3K9ac peptide interaction at 1 uM by AlphaScreen assay | Homo sapiens | 2 | ALA4808215 | single protein format | Scientific Literature | |
15. | ALA4810345 | Binding | Inhibition of human His-ENL YEATS domain and biotinylated H3K9ac peptide interaction at 5 uM by AlphaScreen assay | Homo sapiens | 2 | ALA4808215 | single protein format | Scientific Literature | |
16. | ALA4810353 | Binding | Stabilization of ENL in human MV4-11 cells assessed as inhibition of high temperature-induced ENL protein degradation at 20 uM incubated for 5 hrs by cellular thermal shift assay | Homo sapiens | 1 | ALA4808215 | cell-based format | Scientific Literature | |
17. | ALA4810354 | Binding | Stabilization of ENL in human MOLM13 cells assessed as inhibition of high temperature-induced ENL protein degradation at 20 uM incubated for 5 hrs by cellular thermal shift assay | Homo sapiens | 1 | ALA4808215 | cell-based format | Scientific Literature | |
18. | ALA4810355 | Binding | Stabilization of GAS41 in human MV4-11 cells assessed as inhibition of high temperature-induced GAS41 protein degradation at 20 uM incubated for 5 hrs by cellular thermal shift assay | Homo sapiens | 1 | ALA4808215 | cell-based format | Scientific Literature | |
19. | ALA4810356 | Binding | Stabilization of GAS41 in human MOLM13 cells assessed as inhibition of high temperature-induced GAS41 protein degradation at 20 uM incubated for 5 hrs by cellular thermal shift assay | Homo sapiens | 1 | ALA4808215 | cell-based format | Scientific Literature | |
20. | ALA4810357 | Binding | Stabilization of ENL in human HeLa cells assessed as inhibition of high temperature-induced ENL protein degradation at 20 uM incubated for 5 hrs by cellular thermal shift assay | Homo sapiens | 1 | ALA4808215 | cell-based format | Scientific Literature |