#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4222550	B	Inhibition of human DCN3-mediated cullin neddylation assessed as reduction in transfer of fluorescein-5-maleimide-tagged NEDD8 from N-terminally acetylated human UBE2M to CUL2-CTD at 50 uM measured for 2 mins by pulse-chase assay	Homo sapiens	1		single protein format	Scientific Literature
2.	ALA4222559	B	Inhibition of human DCN3 stimulated N-terminal cys-tagged NEDD8 transfer from UBE2F to CUL2(CTD)-RBX1 at 10 uM measured up to 2 mins by pulse-chase assay	Homo sapiens	2		single protein format	Scientific Literature
3.	ALA4378271	B	Inhibition of DCN3 (unknown origin) assessed as decrease in DCN1-stimulated NEDD8 transfer from UBE2M to CUL2 at 120 uM after 10 sec by pulse-chase assay	Homo sapiens	1		single protein format	Scientific Literature
4.	ALA4398587	B	Binding affinity to N-terminal GST tagged recombinant DCN3 (unknown origin) (86 to 304 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1/FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay	Homo sapiens	1		assay format	Scientific Literature
5.	ALA4817614	B	Inhibition of recombinant human GST-tagged DCN3 (86 to 304 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay	Homo sapiens	1		assay format	Scientific Literature
6.	ALA5043819	B	Binding affinity to DCN3 (unknown origin) assessed as inhibitory constant	Homo sapiens	2		single protein format	Scientific Literature