| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4222551 | B | Inhibition of human DCN4-mediated cullin neddylation assessed as reduction in transfer of fluorescein-5-maleimide-tagged NEDD8 from N-terminally acetylated human UBE2M to CUL2-CTD at 50 uM measured for 1 min by pulse-chase assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 2. | ALA4378272 | B | Inhibition of DCN4 (unknown origin) assessed as decrease in DCN1-stimulated NEDD8 transfer from UBE2M to CUL2 at 60 uM after 10 sec by pulse-chase assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 3. | ALA4398588 | B | Binding affinity to N-terminal GST tagged recombinant DCN4 (unknown origin) (102 to 292 residues) expressed in Escherichia coli BL21(DE3) assessed as reduction in DCN1/FAM-labelled N-terminal acetylated UBE2M (1 to 21 residues) protein-protein interaction after 30 mins by HTRF assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 4. | ALA4817615 | B | Inhibition of recombinant human GST-tagged DCN4 (102 to 292 residues) expressed in Escherichia coli BL21 (DE3) using MIKLFSLKQQKKEEESAGGTK-biotin as substrate incubated for 30 mins by HTRF assay | Homo sapiens | 1 | assay format | Scientific Literature | ||
| 5. | ALA5043820 | B | Binding affinity to DCN4 (unknown origin) assessed as inhibitory constant | Homo sapiens | 2 | single protein format | Scientific Literature |
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