# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4118552 | B | Binding affinity to FUBP1 in human NCI-H23 cells at 1 uM by mass spectrometry based pull down assay | Homo sapiens | 1 | ALA4118021 | cell-based format | Scientific Literature | |
2. | ALA4719415 | B | Inhibition of FUBP1 in human Hep3B cells assessed as upregulation of BIK mRNA expression at 10 uM after 6 hrs by qRT-PCR analysis | Homo sapiens | 2 | ALA4715785 | cell-based format | Scientific Literature | |
3. | ALA4719416 | B | Inhibition of FUBP1 in human Hep3B cells assessed as upregulation of p21 mRNA expression at 10 uM after 6 hrs by qRT-PCR analysis | Homo sapiens | 2 | ALA4715785 | cell-based format | Scientific Literature | |
4. | ALA4719425 | B | Inhibition of human full length FUBP1 expressed in Escherichia coli BL21 DE3 assessed as reduction in FUBP1 interaction with FUSE BIK oligonucleotide at 100 uM incubated for 21 hrs by Alphascreen assay relative to DMSO | Homo sapiens | 1 | ALA4715785 | cell-based format | Scientific Literature | |
5. | ALA4719426 | B | Inhibition of human full length FUBP1 expressed in Escherichia coli BL21 DE3 assessed as reduction in FUBP1 interaction with FUSE p21 oligonucleotide at 100 uM incubated for 21 hrs by Alphascreen assay relative to DMSO | Homo sapiens | 4 | ALA4715785 | cell-based format | Scientific Literature | |
6. | ALA4719427 | B | Inhibition of full length 6xHis-tagged human FUBP1 expressed in HEK293T cells assessed as reduction in FUBP1 interaction with biotinylated NLC chip immobilized FUSE p21 oligonucleotide by measuring time taken for association at 100 uM incubated for 21 hrs by SPR analysis | Homo sapiens | 4 | ALA4715785 | cell-based format | Scientific Literature | |
7. | ALA4719428 | B | Inhibition of 6xHis-tagged human FUBP1 expressed in HEK293T cells assessed as reduction in FUBP1 interaction with biotinylated NLC chip immobilized FUSE p21 oligonucleotide by measuring time taken for dissociation at 100 uM incubated for 21 hrs by SPR analysis | Homo sapiens | 4 | ALA4715785 | cell-based format | Scientific Literature | |
8. | ALA4719429 | B | Inhibition of human full length FUBP1 expressed in Escherichia coli BL21 DE3 assessed as reduction in FUBP1 interaction with FUSE p21 oligonucleotide incubated for 21 hrs by Alphascreen assay | Homo sapiens | 1 | ALA4715785 | cell-based format | Scientific Literature | |
9. | ALA4719430 | B | Inhibition of 6xHis-tagged human FUBP1 expressed in HEK293T cells assessed as reduction in FUBP1 interaction with biotinylated NLC chip immobilized FUSE p21 oligonucleotide incubated for 21 hrs by SPR analysis | Homo sapiens | 32 | ALA4715785 | cell-based format | Scientific Literature | |
10. | ALA4719431 | B | Inhibition of FUBP1 in human Hep3B cells assessed as upregulation of p21 mRNA expression at 50 uM after 6 hrs by qRT-PCR analysis relative to control | Homo sapiens | 1 | ALA4715785 | cell-based format | Scientific Literature | |
11. | ALA4719432 | B | Inhibition of FUBP1 in human Hep3B cells assessed as upregulation of BIK mRNA expression at 50 uM after 6 hrs by qRT-PCR analysis relative to control | Homo sapiens | 1 | ALA4715785 | cell-based format | Scientific Literature | |
12. | ALA4719433 | B | Inhibition of FUBP1 in human Hep3B cells assessed as downregulation of CCND2 mRNA expression at 50 uM after 6 hrs by qRT-PCR analysis relative to control | Homo sapiens | 1 | ALA4715785 | cell-based format | Scientific Literature | |
13. | ALA5153025 | B | Binding affinity to recombinant GST tagged FUBP1 KH3-KH4 domain (unknown origin) assessed as decrease in FUBP1-FUSE DNA bound complex using 5'-IRDye700 tagged TGTATATTCCCTCGGG oligonucleotide as substrate at 30 to 60 uM measured for 30 mins by Gel shift assay relative to control | Homo sapiens | 1 | ALA5150043 | single protein format | Scientific Literature | |
14. | ALA5153026 | B | Binding affinity to recombinant GST tagged FUBP1 KH3-KH4 domain (unknown origin) assessed as decrease in FUBP1-FUSE DNA bound complex using 5'-IRDye700 tagged TATTCCCTGATTTTT oligonucleotide as substrate measured for 30 mins by Gel shift assay | Homo sapiens | 1 | ALA5150043 | single protein format | Scientific Literature |