# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4191647 | B | Inhibition of RGS17 (unknown origin) GAP activity in presence of GTP by malachite green dye based assay | Homo sapiens | 4 | ALA4190223 | single protein format | Scientific Literature | |
2. | ALA4191648 | B | Binding affinity to RGS17 (unknown origin) by ITC method | Homo sapiens | 2 | ALA4190223 | single protein format | Scientific Literature | |
3. | ALA4191650 | B | Inhibition of wild type GST-tagged RGS17 (unknown origin) interaction with biotinylated Galphao after 30 mins in presence of AMF and GDP by AlphaScreen assay | Homo sapiens | 3 | ALA4190223 | single protein format | Scientific Literature | |
4. | ALA4191651 | B | Binding affinity to RGS17 (unknown origin) assessed as change in melting temperature by differential scanning fluorimetry | Homo sapiens | 3 | ALA4190223 | single protein format | Scientific Literature | |
5. | ALA4191652 | B | Inhibition of RGS17 (unknown origin) C117A mutant interaction with biotinylated Galphao after 30 mins in presence of AMF and GDP by AlphaScreen assay | Homo sapiens | 3 | ALA4190223 | single protein format | Scientific Literature | |
6. | ALA4191654 | B | Binding affinity to RGS17 (unknown origin) assessed as covalent adduct formation at 100 uM after 72 hrs by ESI-MS analysis | Homo sapiens | 3 | ALA4190223 | single protein format | Scientific Literature | |
7. | ALA4191655 | B | Binding affinity to RGS17 (unknown origin) assessed as change in chemical shift perturbations by NMR spectroscopic analysis | Homo sapiens | 3 | ALA4190223 | single protein format | Scientific Literature | |
8. | ALA4191656 | B | Inhibition of Galphao interaction with GFP-fused full length RGS17 (unknown origin) expressed in HEK293 cells assessed as disruption of RGS17 localization at cell membranes at 100 uM after 10 mins by confocal microscopic analysis | Homo sapiens | 1 | ALA4190223 | cell-based format | Scientific Literature | |
9. | ALA4191672 | B | Inhibition of RGS17 (unknown origin)-mediated CREB phosphorylation | Homo sapiens | 2 | ALA4190223 | single protein format | Scientific Literature | |
10. | ALA4191674 | B | Binding affinity to RGS17 (unknown origin) assessed as molecular weight of covalent adduct at 100 uM after 72 hrs by ESI-MS analysis (Rvb = 16232 Da) | Homo sapiens | 3 | ALA4190223 | single protein format | Scientific Literature | |
11. | ALA4417801 | B | Inhibition of RGS17 (unknown origin) assessed as reduction in RGS17 interaction with 6x-his-tagged biotinylated Galpha-o interaction by Alpha-screen assay | Homo sapiens | 4 | ALA4417625 | single protein format | Patent Bioactivity Data | |
12. | ALA4417802 | B | Inhibition of RGS17 (unknown origin) in presence of and ATP Galpha-o by Malachite green steady-state GTPase assay | Homo sapiens | 4 | ALA4417625 | single protein format | Patent Bioactivity Data | |
13. | ALA4417803 | B | Binding affinity to RGS17 (unknown origin) by ITC assay | Homo sapiens | 2 | ALA4417625 | single protein format | Patent Bioactivity Data | |
14. | ALA4417804 | B | Binding affinity to RGS17 (unknown origin) assessed as shifting of protein melting temperature by Sypro-orange dye based differential scanning fluorimetry | Homo sapiens | 4 | ALA4417625 | single protein format | Patent Bioactivity Data | |
15. | ALA4417806 | B | Inhibition of RGS17 (unknown origin) assessed as reduction in RGS17 interaction with 6x-his-tagged biotinylated Galpha-o interaction at 100 uM followed by 3 times drug wash out with ASB buffer by Alpha-screen assay | Homo sapiens | 4 | ALA4417625 | single protein format | Patent Bioactivity Data | |
16. | ALA4417807 | B | Inhibition of RGS17 (unknown origin) assessed as reduction in RGS17 interaction with 6x-his-tagged biotinylated Galpha-o interaction at 100 uM by Alpha-screen assay | Homo sapiens | 4 | ALA4417625 | single protein format | Patent Bioactivity Data | |
17. | ALA4417808 | B | Covalent binding affinity to N-terminal deficient RGS17 (unknown origin) assessed as covalent protein-compound adduct formation at 50 uM incubated for 1 hr by MALDI-TOF mass spectrometry | Homo sapiens | 4 | ALA4417625 | single protein format | Patent Bioactivity Data | |
18. | ALA4417809 | B | Inhibition of cell membrane translocation of N-terminal GFP tagged full length RGS17 (unknown origin) expressed in HEK293T cells co-expressing Galpha-o at 100 uM incubated for 10 mins by confocal microscopy | Homo sapiens | 1 | ALA4417625 | cell-based format | Patent Bioactivity Data | |
19. | ALA4417810 | B | Inhibition of cell membrane translocation of N-terminal GFP tagged and N-terminal deficient RGS17 (unknown origin) expressed in HEK293T cells co-expressing Galpha-o assessed as induction of delocalization of RGS17deltaN to cytoplasm at 100 uM incubated for 10 mins by confocal microscopy | Homo sapiens | 5 | ALA4417625 | cell-based format | Patent Bioactivity Data | |
20. | ALA4417812 | B | Inhibition of cell membrane translocation of N-terminal GFP tagged and N-terminal deficient RGS17 (unknown origin) expressed in HEK293T cells co-expressing Galpha-o assessed as induction of delocalization of RGS17deltaN to cytoplasm at 10 uM incubated for 10 mins by confocal microscopy | Homo sapiens | 3 | ALA4417625 | cell-based format | Patent Bioactivity Data |