#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4136598	B	Inhibition of recombinant human His6-tagged USP25 expressed in baculovirus infected Sf21 insect cells using Ub-Rh110 as substrate pretreated for 30 mins followed by substrate addition measured after 45 mins by fluorescence assay	Homo sapiens	8		cell-based format	Scientific Literature
2.	ALA4418452	B	Inhibition of recombinant C-terminal 6-His tagged human USP25 using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 45 mins by fluorescence based assay	Homo sapiens	21		single protein format	Patent Bioactivity Data
3.	ALA4477146	B	Inhibition of recombinant C-terminal 6-His tagged human USP25 using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 45 mins by fluorescence based assay	Homo sapiens	223		single protein format	Patent Bioactivity Data
4.	ALA4605984	B	Inhibition of human GST-tagged USP25 expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis	Homo sapiens	1		assay format	Scientific Literature
5.	ALA4674637	B	Inhibition of USP25 (unknown origin) at 10 uM relative to control	Homo sapiens	2		single protein format	Scientific Literature
6.	ALA4687440	B	Inhibition of USP25 (unknown origin) at 20 uM	Homo sapiens	2		single protein format	Scientific Literature
7.	ALA5153783	B	Inhibition of USP25 (unknown origin) at 10 uM using ubiquitin-rhodamine 110 as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by microplate spectrophotometer analysis	Homo sapiens	6		single protein format	Scientific Literature
8.	ALA5234108	B	Inhibition of USP25 (unknown origin) using Ub-Rho100 as substrate by calorimetric analysis	Homo sapiens	3		single protein format	Scientific Literature
9.	ALA5251161	B	Inhibition of USP25 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay	Homo sapiens	2		single protein format	Scientific Literature