#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA761790	B	Inhibition of DNA repair Poly (ADP-ribose) polymerase 1 in permeabilised L1210 murine leukemia; NT =' not tested'	Mus musculus	1	ALA1131437	single protein format	Scientific Literature
2.	ALA761791	B	Inhibition of DNA repair Poly (ADP-ribose) polymerase 1 in permeabilised L1210 murine leukemia cells; NT = not tested	Mus musculus	12	ALA1131437	cell-based format	Scientific Literature
3.	ALA761792	B	Inhibition of the DNA repair Poly (ADP-ribose) polymerase 1, in permeabilised L1210 murine leukemia cells	Mus musculus	28	ALA1131437	cell-based format	Scientific Literature
4.	ALA761793	B	Inhibition of DNA repair Poly (ADP-ribose) polymerase 1 in permeabilised L1210 murine leukemia cells; not tested	Mus musculus	1	ALA1131437	cell-based format	Scientific Literature
5.	ALA763196	B	Tested for inhibition of poly(ADP-ribose) polymerase-1 (PARP-1) in mouse using Cell protection assay		29	ALA1146715	single protein format	Scientific Literature
6.	ALA763197	B	Tested for inhibition of poly(ADP-ribose) polymerase-1 (PARP-1) in mouse using Cell protection assay; nd = not determined		1	ALA1146715	single protein format	Scientific Literature
7.	ALA763198	B	Percent inhibition of poly(ADP-ribose) polymerase-1 (PARP-1) in mouse using Cell protection assay at 10 uM		6	ALA1146715	single protein format	Scientific Literature
8.	ALA763199	B	Percent inhibition of poly(ADP-ribose) polymerase-1 (PARP-1) in mouse using Cell protection assay at 10 uM; nd = not determined		1	ALA1146715	single protein format	Scientific Literature
9.	ALA2039743	B	Inhibition of PARP1 in MEF cells assessed as potentiation of MMC-induced cytotoxicity at 0.01 uM incubated for 1 hr by clonogenic survival assay relative to untreated control	Mus musculus	1	ALA2034898	cell-based format	Scientific Literature
10.	ALA3705444	B	Biochemical Assay: The assay is based on the use of a probe of formula (IP) that binds to the NAD binding pocket and takes advantage of the significant change in the polarization signal observed upon binding of the probe to PARP-1, -2 and -3.The probe of formula (IP) was tested for its ability to bind FL PARP-1, -2 and -3 in a titration experiment. The assay performances were then evaluated (Zâ factor) as well as the displacement of the probe by its scaffold and known commercially available PARP inhibitors. In all of the experiments, the polarization signal was measured using a Saphire2 plate reader (Tecan). Data analysis was performed using Dynafit software. In particular, titration data were fitted to the following equilibria: Enzyme+probe <==> Complex Enzyme-probe, while displacement data were fitted to the following equilibria: Enzyme+probe <==> Complex Enzyme-probe, Enzyme+Compound <==> Complex Enzyme-Compound, whereby binding of probe and compound on the enzyme are mutually exclusive.	Mus musculus	48	ALA3639114	single protein format	BindingDB Database
11.	ALA3705445	B	Biochemical Assay: Affinity evaluation of the tested compounds and their selectivity with respect to the different PARP isoforms of interest was assessed in a displacement assay.The identification of compounds capable of binding several PARP proteins is carried out through a screening method including the steps ofa) providing a reaction mixture containing:the PARP protein isoform under investigation,a compound of formula (IP): wherein R10 is hydrogen or methyl, B is (CH2)nNH group, n is 2 to 6; m is 0 or 1 and X− is a counterion, and serial dilutions of the test compound;b) comparing the polarization signal generated in the absence of the test compound with the one generated in the presence of different concentrations of the test compound, andc) evaluating the ability of the test compound to displace the compound of formula (IP) as defined above indicated from a decreased fluorescence polarization level. The polarization signal can be measured, e.g., by a plate reader such as the Saphire2 (Tecan).	Mus musculus	46	ALA3639114	single protein format	BindingDB Database
12.	ALA5253421	B	Inhibition of mouse recombinant PARP-1 incubated for 60 mins	Mus musculus	1	ALA5252527	single protein format	Scientific Literature