# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4219635 | B | Inhibition of His-tagged VHL/elongin B/elongin C (unknown origin) interaction with HIF1alpha preincubated for 10 mins followed by fluorescin labelled HIF1alpha peptide addition measured after 2 hrs by fluorescence polarization assay | Homo sapiens | 9 | ALA4219031 | protein complex format | Scientific Literature | |
2. | ALA4234786 | B | Binding affinity to N-terminal His6 tagged VHL (54 to 213 residues)/ELoC (17 to 112 residues)/EloB (1 to 120 residues)/HIF-1alpha (unknown origin) complex expressed in Escherichia coli BL21(DE3) assessed as change in melting temperature at 1 mM in presence of DEALA-Hyp-YIPMDDDFQLRSF by NMR analysis | Homo sapiens | 2 | ALA4229472 | assay format | Scientific Literature | |
3. | ALA4234788 | B | Binding affinity to N-terminal His6 tagged VHL (54 to 213 residues)/ELoC (17 to 112 residues)/EloB (1 to 120 residues)/HIF-1alpha (unknown origin) complex expressed in Escherichia coli BL21(DE3) assessed as change in melting temperature at 1 mM by Sypro orange dye based differential scanning fluorimetric analysis | Homo sapiens | 3 | ALA4229472 | assay format | Scientific Literature |