| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA5249914 | B | Protac activity at VHL/BRD4 (unknown origin) | Homo sapiens | 1 | protein format | Scientific Literature | ||
| 2. | ALA5249915 | B | Protac activity at VHL/BRD4 in human HCT-116 cells assessed as degradation of BRD4 incubated for 24 hrs by immunoblot assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 3. | ALA5249917 | B | Protac activity at VHL/BRD4 in human HeLa cells assessed as degradation of BRD4 incubated for 24 hrs by Western blotting analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 4. | ALA5249928 | B | Protac activity at VHL/BRD4 in HEK293 cells assessed as degradation of BRD4 incubated for 4 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4190493 | B | Proteolysis targeting chimera activity in human 22Rv1 cells assessed as induction of VHL-mediated delivery of BRD4 for protein degradation by proteasome by measuring drug level causing 50% substrate depletion incubated for 16 hrs by Western blot method | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4219415 | B | Proteolysis targeting chimera activity in human NCI-H661 cells assessed as induction of VHL-mediated delivery of BRD4 for protein degradation by proteasome after 4 hrs in presence of 2-(prop-2-ynyloxy)ethanol by Meso Scale Diagnostics assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4219417 | B | Induction of human His-tagged VHL(1 to 154)/GST-tagged BRD4 (49 to 170 residues) interaction assessed as VHL/BRD4/compound complex formation preincubated for 60 mins and measured after 60 mins by luminescence based Alphascreen proximity assay relative to control | Homo sapiens | 6 | protein format | Scientific Literature | ||
| 8. | ALA4219419 | B | Proteolysis targeting chimera activity in human HeLa cells assessed as induction of VHL-mediated delivery of BRD4 for protein degradation by proteasome by measuring drug level causing 50% cellular protein depletion incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4349411 | B | Protac activity against VHL/BRD4 in human LNCaP95 cells assessed as induction of BRD4 protein degradation by reverse phase protein array analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 10. | ALA4349414 | B | Protac activity against VHL/BRD4 (unknown origin) assessed as induction of BRD4 protein degradation by cell based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4388589 | B | Protac activity at VHL/BRD4 in human HeLa cells assessed as induction of BRD4 protein degradation incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4388590 | B | Protac activity at VHL/BRD4 in human HeLa cells assessed as induction of BRD4 protein degradation at 50 nM up to 36 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 13. | ALA4388591 | B | Protac activity at VHL/BRD4 in human 22Rv1 cells assessed as induction of BRD4 protein degradation incubated for 16 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 14. | ALA4388592 | B | Protac activity at VHL/BRD4 in human 22Rv1 cells assessed as induction of BRD4 protein degradation incubated for 16 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA5036172 | B | Protac activity at VHL/Brd4 in HEK293 cells assessed as Brd4 degradation after 4 hrs by Western blotting analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 16. | ALA5036178 | B | Binding affinity to N-terminal His6-tagged VHL (54 to 213 residues)/BRD4 BD2 (333 to 460 residues) (unknown origin) expressed in Escherichia coli assessed as displacement of HIF-1alpha preincubated with Brd4 BD2 by ternary binding competitive fluorescence polarization assay | Homo sapiens | 8 | assay format | Scientific Literature | ||
| 17. | ALA5129898 | B | PROTAC activity at VHL/BRD4 in human MCF7 cells assessed as BRD4 degradation measured after 24 hrs by Western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 18. | ALA5129899 | B | PROTAC activity at VHL/BRD4 in human MCF7 cells assessed as BRD4 degradation by measuring maximum efficacy at 50 nM measured after 24 hrs by Western blot analysis relative to control | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 19. | ALA5164200 | B | PROTAC activity at VHL/BRD4 in human MV4-11 cells assessed as induction of BRD4 degradation at 1 uM measured after 24 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 20. | ALA5164201 | B | PROTAC activity at VHL/BRD4 in human MV4-11 cells assessed as induction of BRD4 degradation at 3 to 100 nM measured after 24 hrs by western blot analysis | Homo sapiens | 1 | cell-based format | Scientific Literature |
The page will load shortly, Thanks for your patience!