Proteolysis targeting chimera activity in human MV4-11 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular protein depletion at 50 nM incubated for 4 hrs by Western blotting method
Proteolysis targeting chimera activity in human MV4-11 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular cMyc protein depletion at 50 nM incubated for 4 hrs by Western blotting method
Proteolysis targeting chimera activity in human HL60 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular protein depletion at 50 nM incubated for 4 hrs by Western blotting method
Proteolysis targeting chimera activity in human HL60 cells assessed as induction of VCB-mediated delivery of BRD4 for protein degradation by proteasome by measuring cellular cMyc protein depletion at 50 nM incubated for 4 hrs by Western blotting method
Induction of interaction of N-terminal His6-tagged recombinant human VHL (54 to 213 residues)/elongin C (17 to 112 residues)/elongin B (1 to 104 residues) co-expressed in Escherichia coli BL21 (DE3) with recombinant human Brd4 BD2 assessed as VCB/BRD4/compound ternary complex formation by isothermal titration calorimetry-based assay