| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4219432 | B | Induction of cereblon-mediated GSPT1 degradation in human DF15 cells expressing ePL-tagged GSPT1 at 0.0005 to 10 uM after 4 hrs by luminometric analysis | Homo sapiens | 1 | ALA4219026 | cell-based format | Scientific Literature | |
| 2. | ALA4323258 | B | Inhibition of cereblon binding with GSPT1 in human MDA-MB-231 cells assessed as decrease in GSPT1 degradation at 0.1 uM after 2 hrs in presence of 30 uM lenalidomide by Western blot analysis | Homo sapiens | 1 | ALA4321819 | cell-based format | Scientific Literature | |
| 3. | ALA4323259 | B | Inhibition of cereblon binding with GSPT1 human MDA-MB-468 cells assessed as decrease in GSPT1 degradation at 0.1 uM after 2 hrs in presence of 30 uM lenalidomide by Western blot analysis | Homo sapiens | 1 | ALA4321819 | cell-based format | Scientific Literature | |
| 4. | ALA4628557 | B | Induction of CRBN-mediated pLOC-ePL-tagged GSPT1 (unknown origin) degradation in human DF15 cells assessed as minimum protein remaining incubated for 4 hrs by luminescence based beta-galactosidase enzyme fragmentation complementation assay | Homo sapiens | 14 | ALA4627320 | cell-based format | Scientific Literature | |
| 5. | ALA4628558 | B | Induction of CRBN-mediated pLOC-ePL-tagged GSPT1 (unknown origin) degradation in human DF15 cells assessed as minimum protein remaining up to 10 uM incubated for 4 hrs by luminescence based beta-galactosidase enzyme fragmentation complementation assay relative to control | Homo sapiens | 2 | ALA4627320 | cell-based format | Scientific Literature | |
| 6. | ALA4712276 | B | Protac activity at CRBN/GSPT1 in human HT-29 cells assessed as induction of GSPT1 degradation up to 10 uM incubated for 24 hrs by Western blot analysis | Homo sapiens | 1 | ALA4706631 | cell-based format | Scientific Literature | |
| 7. | ALA4714171 | B | Protac activity at CRBN/GSPT1 in human NB-4 cells assessed as induction of GSPT1 degradation at 0.001 to 1 uM incubated for 4 hrs by western blot analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 8. | ALA4714184 | B | Protac activity at CRBN/GSPT1 in human KG-1 cells assessed as GSPT1 degradation at 1 uM incubated for 4 hrs in presence of proteasome inhibitor bortezomib by western blot analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 9. | ALA4714185 | B | Protac activity at CRBN/GSPT1 in human KG-1 cells assessed as GSPT1 degradation at 1 uM incubated for 4 hrs in presence of neddylation inhibitor MLN4924 by western blot analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 10. | ALA4714186 | B | Protac activity at CRBN/GSPT1 in human KG-1 cells assessed as GSPT1 degradation by measuring accumulation of ATF4 at 200 uM incubated for 4 hrs by western blot analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 11. | ALA4714187 | B | Protac activity at CRBN/GSPT1 in human KG-1 cells assessed as GSPT1 degradation by measuring accumulation of ATF3 at 200 uM incubated for 4 hrs by western blot analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 12. | ALA4714188 | B | Protac activity at CRBN/GSPT1 in human KG-1 cells assessed as GSPT1 degradation by measuring accumulation of CHOP at 200 uM incubated for 4 hrs by western blot analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 13. | ALA4714189 | B | Protac activity at CRBN/GSPT1 in human DF15 cells assessed as GSPT1 degradation incubated for 4 hrs by hunter detection reagent based luminescence assay | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 14. | ALA4714190 | B | Protac activity at CRBN/GSPT1 in human DF15 cells assessed as maximum GSPT1 degradation incubated for 4 hrs by hunter detection reagent based luminescence assay relative to control | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 15. | ALA4714191 | B | Protac activity at CRBN/GSPT1 in human DF-15 cells assessed as maximum GSPT1 degradation incubated for 20 hrs by hunter detection reagent based luminescence assay relative to control | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 16. | ALA4714194 | B | Protac activity at CRBN/GSPT1 in human DF-15 cells assessed as GSPT1 degradation at 0.2 to 3 uM incubated for 4 to 6 hrs by Incell-hunter detection reagent based luminescence assay relative to control | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 17. | ALA4714197 | B | Protac activity at CRBN/GSPT1 in CD3+ T cells derived from patients assessed as reduction of GSPT1 levels in peripheral blood blast administered intravenously for 5 days by flow cytometry analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 18. | ALA4714222 | B | Protac activity at CRBN/GSPT1 in CD3+ T cells derived from patients assessed as reduction of GSPT1 levels at 2.4 mg, iv administered for 5 days by flow cytometry analysis relative to control | Homo sapiens | 1 | ALA4706692 | organism-based format | Scientific Literature | |
| 19. | ALA4714224 | B | Protac activity at CRBN/GSPT1 in CD3+ T cells derived from patients assessed as reduction of GSPT1 levels in T cells administered intravenously for 5 days by flow cytometry analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature | |
| 20. | ALA4714225 | B | Protac activity at CRBN/GSPT1 in human KG-1 cells assessed as GSPT1 degradation at 1 uM incubated for 4 hrs by western blot analysis | Homo sapiens | 1 | ALA4706692 | cell-based format | Scientific Literature |
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