| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4392309 | B | Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control | Cryptosporidium hominis | 33 | assay format | Scientific Literature | ||
| 2. | ALA4392310 | B | Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as decrease in TS activity | Cryptosporidium hominis | 1 | assay format | Scientific Literature | ||
| 3. | ALA4392312 | B | Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as substrate Kcat at 100 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 2.17 +/- 0.04 /s) | Cryptosporidium hominis | 1 | assay format | Scientific Literature | ||
| 4. | ALA4392313 | B | Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as substrate Kcat at 250 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 2.17 +/- 0.04 /s) | Cryptosporidium hominis | 1 | assay format | Scientific Literature | ||
| 5. | ALA4392314 | B | Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as substrate Km at 100 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 1.2 +/- 0.2 uM) | Cryptosporidium hominis | 1 | assay format | Scientific Literature | ||
| 6. | ALA4392315 | B | Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as substrate Km at 250 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 1.2 +/- 0.2 uM) | Cryptosporidium hominis | 1 | assay format | Scientific Literature | ||
| 7. | ALA4392316 | B | Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as Kobs at 100 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 1.98 +/- 0.09 /s) | Cryptosporidium hominis | 1 | assay format | Scientific Literature | ||
| 8. | ALA4392317 | B | Mixed noncompetitive inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as Kobs at 250 uM using varying levels of [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by Lineweaver-Burk plot analysis (Rvb = 1.98 +/- 0.09 /s) | Cryptosporidium hominis | 1 | assay format | Scientific Literature | ||
| 9. | ALA4392318 | B | Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity using [3',5',7,9-3H]-H2folate as substrate preincubated for 10 mins before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control | Cryptosporidium hominis | 6 | assay format | Scientific Literature | ||
| 10. | ALA4392319 | B | Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 1 hr to 72 hrs before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control | Cryptosporidium hominis | 2 | assay format | Scientific Literature | ||
| 11. | ALA4392320 | B | Inhibition of recombinant Cryptosporidium hominis TS-DHFR Cys44Ser mutant expressed in Escherichia coli PA414 assessed as reduction in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 16 hrs before NADPH addition for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control | Cryptosporidium hominis | 3 | assay format | Scientific Literature | ||
| 12. | ALA4392322 | B | Inhibition of recombinant Cryptosporidium hominis TS-DHFR expressed in Escherichia coli PA414 assessed as decrease in DHFR steady state activity at 500 uM using [3',5',7,9-3H]-H2folate as substrate preincubated for 16 hrs with DTT prior to NADPH addition and incubated for 5 mins followed by substrate addition and measured after 5 mins by NADPH based radiometric chemical quench assay relative to control | Cryptosporidium hominis | 3 | assay format | Scientific Literature | ||
| 13. | ALA4409185 | B | Inhibition of recombinant Cryptosporidium hominis TS-DHFR expresssed in Escherichia coli PA414 using dUMP and 5,10-methylenetetrahydrofolate as substrate by spectrometry | Cryptosporidium hominis | 26 | single protein format | Scientific Literature |
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