# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA807606 | F | Rate of phosphorylation of labeled sphingosine derivative by human SPHK-1 expressed in HEK293 cells relative to rate of D-sphingosine | 8 | cell-based format | Scientific Literature | |||
2. | ALA864526 | B | Rate of phosphorylation by recombinant human SPHK1 | Homo sapiens | 6 | single protein format | Scientific Literature | ||
3. | ALA902398 | B | Effect on rate of phosphorylation of human recombinant SPHK1 expressed in HEK293 cells | Homo sapiens | 4 | cell-based format | Scientific Literature | ||
4. | ALA2015530 | B | Inhibition of SPHK1 at 10 uM | 1 | single protein format | Scientific Literature | |||
5. | ALA1821102 | B | Inhibition of N-terminal GST-fused sphingosine kinase 1 (1-384) expressed in baculovirus expression system assessed as inhibition of sphingosine phosphorylation after 1 hr by off-chip mobility shift assay | 9 | assay format | Scientific Literature | |||
6. | ALA1962074 | B | GSK_PKIS: SPHK1 mean inhibition at 0.1 uM [Nanosyn] | 366 | single protein format | GSK Published Kinase Inhibitor Set | |||
7. | ALA1962075 | B | GSK_PKIS: SPHK1 mean inhibition at 1 uM [Nanosyn] | 366 | single protein format | GSK Published Kinase Inhibitor Set | |||
8. | ALA952612 | B | Inhibition of Sphk1 expressed in HEK293 cells | 1 | cell-based format | Scientific Literature | |||
9. | ALA995442 | B | Inhibition of EGFP-fused human SPHK1 expressed in CHO cells at 10 uM | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
10. | ALA995444 | B | Inhibition of EGFP-fused human SPHK1 expressed in CHO cells using D-erythro-sphingosine as substrate by Michaelis-Menten plot | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
11. | ALA995446 | B | Inhibition of EGFP-fused human SPHK1 expressed in CHO cells | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
12. | ALA995447 | B | Effect on EGFP-fused human SPHK1 activity expressed in CHO cells at 10 uM relative to basal level | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
13. | ALA995451 | B | Inhibition of human SPHK1 expressed in human U937 cells assessed as inhibition of endogenous sphingosine-1-phosphate levels at 5 uM after 2 hrs by ELISA | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
14. | ALA1032133 | B | Inhibition of SphK1 | 8 | single protein format | Scientific Literature | |||
15. | ALA1051862 | B | Activity at human SPHK1 at 100 uM | Homo sapiens | 1 | single protein format | Scientific Literature | ||
16. | ALA1052796 | B | Inhibition of human sphingosine kinase 1 by off chip mobility shift assay | Homo sapiens | 27 | single protein format | Scientific Literature | ||
17. | ALA1105940 | B | Inhibition of SPHK1 at 5 uM | 1 | single protein format | Scientific Literature | |||
18. | ALA1111694 | B | Inhibition of human recombinant SPHK1 expressed in baculovirus-infected Sf9 cells in presence of sphingosine | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
19. | ALA1111705 | B | Ratio of Kinact to Km for human recombinant SPHK1 expressed in baculovirus-infected Sf9 cells | Homo sapiens | 14 | cell-based format | Scientific Literature | ||
20. | ALA1211760 | B | Inhibition of sphingosine kinase 1 | 29 | single protein format | Scientific Literature |