| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4379082 | B | Binding affinity to DNMT2 (unknown origin) at protein to compound concentration ratio of 1:20 by differential scanning fluorimetry | Homo sapiens | 1 | ALA4376834 | single protein format | Scientific Literature | |
| 2. | ALA5136927 | B | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM incubated for 5 mins by microscale thermophoresis assay | Homo sapiens | 38 | ALA5131574 | assay format | Scientific Literature | |
| 3. | ALA5136928 | B | Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM using tRNA asp as substrate and SAM as co-substrate incubated for 5 mins by tritium incorporation assay relative to control | Homo sapiens | 38 | ALA5131574 | assay format | Scientific Literature | |
| 4. | ALA5136929 | B | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as dissociation constant at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
| 5. | ALA5136930 | B | Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
| 6. | ALA5136932 | B | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as gibbs free energy change at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
| 7. | ALA5136933 | B | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as change in enthalpy change at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
| 8. | ALA5136934 | B | Binding affinity to full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS assessed as change in entropy at 100 uM by isothermal titration calorimetry assay | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
| 9. | ALA5136939 | B | Inhibition of full length human N-terminal his6-tagged DNMT2 expressed in Escherichia coli Rosetta2(DE3)pLysS at 100 uM by liquid scintillation counting analysis | Homo sapiens | 6 | ALA5131574 | assay format | Scientific Literature | |
| 10. | ALA5136942 | B | Inhibition of DNMT2-mediated tRNA methylation in human HEK293 cells assessed as reduction in m5C at C38 of tRNA at 100 uM incubated for 24 hrs by LC-MS/MS analysis | Homo sapiens | 2 | ALA5131574 | cell-based format | Scientific Literature | |
| 11. | ALA5136943 | B | Inhibition of DNMT2-mediated tRNA methylation in human HEK293 cells assessed as reduction in m5C at C38 of tRNA by measuring m5C level at 100 uM incubated for 24 hrs by bisulfite sequencing analysis | Homo sapiens | 2 | ALA5131574 | cell-based format | Scientific Literature | |
| 12. | ALA5252473 | B | Inhibition of full-length human DNMT2 expressed in bacteria using tRNA-Asp as substrate and 3H-SAM as cosubstrate by tritium incorporation assay | Homo sapiens | 29 | ALA5247681 | assay format | Scientific Literature | |
| 13. | ALA5252474 | B | Binding affinity to human DNMT2 at 100 uM by tritium incorporation assay relative to control | Homo sapiens | 1 | ALA5247681 | single protein format | Scientific Literature | |
| 14. | ALA5252475 | B | Displacement of FTAD from full-length human DNMT2 expressed in bacteria at 20 uM incubated for 10 mins by MST assay relative to control | Homo sapiens | 30 | ALA5247681 | assay format | Scientific Literature | |
| 15. | ALA5252476 | B | Displacement of FTAD from full-length human DNMT2 expressed in Escherichia coli incubated for 10 mins by MST assay | Homo sapiens | 1 | ALA5247681 | assay format | Scientific Literature | |
| 16. | ALA5252477 | B | Displacement of FTAD from full-length human DNMT2 expressed in bacteria incubated for 10 mins by MST assay | Homo sapiens | 29 | ALA5247681 | assay format | Scientific Literature | |
| 17. | ALA5252478 | B | Binding affinity to full-length human DNMT2 expressed in bacteria assessed as apparent dissociation constant by ITC method | Homo sapiens | 29 | ALA5247681 | assay format | Scientific Literature | |
| 18. | ALA5252479 | B | Inhibition of full-length human DNMT2 expressed in bacteria using tRNA-Asp as substrate and [3H[-SAM as cosubstrate at 100 uM by tritium incorporation assay relative to control | Homo sapiens | 29 | ALA5247681 | assay format | Scientific Literature | |
| 19. | ALA5252480 | B | Inhibition of full-length human DNMT2 expressed in bacteria in presence of DTT by tritium incorporation assay | Homo sapiens | 1 | ALA5247681 | assay format | Scientific Literature | |
| 20. | ALA5252485 | B | Binding affinity to human DNMT2 deletion mutant expressed in Escherichia coli Rosetta 2 (DE3) pLysS cells incubated for 1 hr under denaturing condition by protein mass spectrometry | Homo sapiens | 2 | ALA5247681 | cell-based format | Scientific Literature |
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