# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4482652 | Binding | Inhibition of Omi/HtrA2 (unknown origin)-induced caspase-independent apoptosis in caspase 9 (-/-) fibroblasts transfected with Omi-GFP up to 10 uM incubated for 36 hrs by propidium iodide and 4',6-diamidino-2-phenylindole staining based fluorescence microscopy relative to control | Homo sapiens | 1 | ALA4482313 | assay format | Patent Bioactivity Data | |
2. | ALA4482653 | Binding | Inhibition of MBP-fused Omi/HtrA2 (134 to 458 residues) (unknown origin) expressed in TB1 Escherichia coli at 20 to 100 uM pre-incubated for 15 mins before FITC-labeled dephosphorylated casein substrate addition and measured every 5 mins for 30 mins by fluorescence assay | Homo sapiens | 4 | ALA4482313 | assay format | Patent Bioactivity Data | |
3. | ALA4482654 | Binding | Inhibition of MBP-fused Omi/HtrA2 (134 to 458 residues) (unknown origin) expressed in TB1 Escherichia coli at 20 uM pre-incubated for 15 mins before FITC-labeled dephosphorylated casein substrate addition and measured every 5 mins for 30 mins by fluorescence assay relative to control | Homo sapiens | 2 | ALA4482313 | assay format | Patent Bioactivity Data | |
4. | ALA4482655 | Binding | Irreversible inhibition of MBP-fused Omi/HtrA2 (134 to 458 residues) (unknown origin) expressed in TB1 Escherichia coli at 20 to 100 uM pre-incubated for 40 mins to 1 hr before FITC-labeled dephosphorylated casein substrate addition and measured every 5 mins for 30 mins by fluorescence assay | Homo sapiens | 4 | ALA4482313 | assay format | Patent Bioactivity Data | |
5. | ALA4482656 | Binding | Inhibition of MBP-fused Omi/HtrA2 (134 to 458 residues) (unknown origin) expressed in TB1 Escherichia coli at 10 uM pre-incubated for 15 mins before higher 2 times FITC-labeled dephosphorylated casein substrate addition and measured every 5 mins for 30 mins by fluorescence assay | Homo sapiens | 1 | ALA4482313 | assay format | Patent Bioactivity Data | |
6. | ALA4482657 | Binding | Inhibition of His-tagged Omi/HtrA2 (134 to 458 residues) (unknown origin) expressed in TB1 Escherichia coli at 10 to 100 uM pre-incubated for 10 mins before un-labeled beta-casein substrate addition and measured after 30 mins by Coomassie blue staining based SDS-PAGE analysis | Homo sapiens | 2 | ALA4482313 | assay format | Patent Bioactivity Data | |
7. | ALA4482658 | Binding | Inhibition of His-tagged Omi/HtrA2 (134 to 458 residues) (unknown origin) expressed in TB1 Escherichia coli at 80 uM pre-incubated for 10 mins before un-labeled beta-casein substrate addition and measured after 30 mins by Coomassie blue staining based SDS-PAGE analysis | Homo sapiens | 1 | ALA4482313 | assay format | Patent Bioactivity Data | |
8. | ALA4482659 | Binding | Inhibition of His-tagged Omi/HtrA2 (134 to 458 residues) (unknown origin) expressed in TB1 Escherichia coli at 10 to 50 uM pre-incubated for 15 mins before FITC-labeled dephosphorylated casein substrate addition and measured every 5 mins for 30 mins by fluorescence assay | Homo sapiens | 1 | ALA4482313 | assay format | Patent Bioactivity Data | |
9. | ALA5258709 | Binding | Binding affinity to recombinant human HTRA2 assessed as increase in fluorescence using H2-OPT as substrate at 1 uM by SDS-PAGE based gel fluorescence scanning analysis | Homo sapiens | 9 | ALA5257069 | single protein format | Scientific Literature | |
10. | ALA5258719 | Binding | Inhibition of recombinant human HTRA2 using H2-OPT as substrate assessed as increase in fluorescence and measured for 2 hrs by fluorescent plate reader analysis | Homo sapiens | 10 | ALA5257069 | single protein format | Scientific Literature | |
11. | ALA5258721 | Binding | Inhibition of recombinant human HTRA2 using H2-OPT as substrate assessed as increase in fluorescence at 1 uM and measured for 2 hrs by fluorescent plate reader analysis | Homo sapiens | 1 | ALA5257069 | single protein format | Scientific Literature |