| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4321135 | B | Inhibition of human IRAP using L-leucine 7-amido-4-methyl coumarin substrate by microplate fluorescence reader based assay | Homo sapiens | 3 | single protein format | Scientific Literature | ||
| 2. | ALA4358835 | B | Activation of IRAP (unknown origin)-mediated Leu-AMC substrate hydrolysis after 60 mins by Rapidfire-MS analysis | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 3. | ALA4372786 | B | Inhibition of human IRAP at <200 uM preincubated for 20 mins followed by L-leucine-7-amido-4-methylcoumarin addition by fluorescence assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
| 4. | ALA4410790 | B | Inhibition of human recombinant IRAP expressed in baculovirus infected Sf9 cells using omniMMP fluorogenic substrate incubated for 5 to 10 mins by fluorescence spectrophotometry analysis | Homo sapiens | 10 | cell-based format | Scientific Literature | ||
| 5. | ALA4410793 | B | Competitive inhibition of human recombinant IRAP expressed in baculovirus infected sf9 cells using L-AMC as substrate incubated for 5 to 10 mins by Michaelis-Menten analysis | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 6. | ALA4410794 | B | Competitive inhibition of human recombinant IRAP expressed in baculovirus infected sf9 cells assessed as substrate Km using varying levels of L-AMC as substrate incubated for 5 to 10 mins by Michaelis-Menten analysis (Rvb = 27 +/- 3 uM) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4410795 | B | Competitive inhibition of human recombinant IRAP expressed in baculovirus infected sf9 cells assessed as substrate Kcat using varying levels of L-AMC as substrate incubated for 5 to 10 mins by Michaelis-Menten analysis (Rvb = 0.10 +/- micromol/min/nmol) | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4625766 | B | Inhibition of human IRAP soluble domain expressed in HEK293S GnTI(-) cells using Arg-AMC as substrate incubated for 2 hrs by fluorescence based assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4625767 | B | Inhibition of human IRAP soluble domain expressed in HEK293S GnTI(-) cells using Leu-AMC as substrate incubated for 2 hrs by fluorescence based assay | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
| 10. | ALA4625770 | B | Competitive inhibition of human IRAP soluble domain expressed in HEK293S GnTI(-) cells assessed as increase in Km with constant Vmax using Arg-AMC as substrate incubated for 2 hrs by fluorescence assay based Michaelis-Menten analysis | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 11. | ALA4699775 | B | Inhibition of recombinant full length human IRAP expressed in HEK293T cells assessed as substrate cleavage using Leu-MCA as substrate by fluorogenic assay | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 12. | ALA4699776 | B | Binding affinity to recombinant full length human IRAP expressed in HEK293T cells using Leu-MCA as substrate | Homo sapiens | 8 | cell-based format | Scientific Literature | ||
| 13. | ALA4699777 | B | Binding affinity to recombinant full length human IRAP expressed in HEK293T cells assessed as binding free energy | Homo sapiens | 13 | cell-based format | Scientific Literature | ||
| 14. | ALA4820027 | B | Inhibition of recombinant human IRAP expressed in HEK293 cells using L-AMC as substrate preincubated for 30 mins followed by substrate addition measured every 3 mins for 1 hr by flourescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
| 15. | ALA4820028 | B | Inhibition of recombinant human IRAP expressed in HEK293 cells at 100 uM using L-AMC as substrate preincubated for 30 mins followed by substrate addition measured every 3 mins for 1 hr by flourescence assay relative to control | Homo sapiens | 3 | cell-based format | Scientific Literature | ||
| 16. | ALA5149507 | B | Inhibition of recombinant IRAP (unknown origin) expressed in HEK293S cells using L-AMC as substrate by flourescence assay | Homo sapiens | 36 | cell-based format | Scientific Literature | ||
| 17. | ALA5149508 | B | Inhibition of recombinant IRAP (unknown origin) expressed in ovalbumin expressing wild type HEK293S cells assessed as decrease in antigen-mediated IL-2 production increase in B3Z T cells pretreated with compound for 1 hr followed by washout and incubated with compound for overnight prior to B3Z T cell addition and measured after 24 hrs by sandwich ELISA assay | Homo sapiens | 2 | cell-based format | Scientific Literature |
The page will load shortly, Thanks for your patience!