#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4418059	B	Inhibition of DNA2 in HU-treated human A549 cells assessed as inhibition of DNA replication fork restart by measuring restarting forks levels at 20 uM pre-incubated for 40 mins followed by incubation with 250 uM C1dU for 40 mins by DNA fiber assay (Rvb = 80%)	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
2.	ALA4418060	B	Inhibition of DNA2 in gammaH2AX-positive human U2OS cells assessed as suppression of DSB repair end resection by measuring reduction in CPT-induced RPA2 phosphorylation at S33 or S4/8 residues	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
3.	ALA4418065	B	Inhibition of nuclease activity of flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells at 250 uM using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method	Homo sapiens	38	ALA4417634	cell-based format	Patent Bioactivity Data
4.	ALA4418066	B	Inhibition of DNA dependent ATPase activity of flag-tagged recombinant DNA2 D294A mutant (unknown origin) expressed in 293T cells using flap DNA substrate	Homo sapiens	38	ALA4417634	cell-based format	Patent Bioactivity Data
5.	ALA4418093	B	Competitive inhibition of nuclease activity of flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method based Lineweaver-Burk plot analysis	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
6.	ALA4418094	B	Inhibition of DNA dependent ATPase activity of flag-tagged recombinant DNA2 D294A mutant (unknown origin) expressed in 293T cells at 1 to 100 uM using flap DNA substrate	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
7.	ALA4418095	B	Binding affinity to flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation at 1 to 1000 uM using flap DNA substrate by EMSA	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
8.	ALA4418096	B	Binding affinity to flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation using flap DNA substrate by EMSA	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
9.	ALA4418097	B	Inhibition of helicase activity of flag-tagged recombinant DNA2 D277A mutant (unknown origin) expressed in 293T cells assessed as remaining 5' to 3' end-dependent DNA helicase activity at 100 mM using M13 phage DNA hybridized to 5'-tailed oligonucleotide substrate and [g-32P]ATP pre-incubated with 5 nM DNA2 enzyme for 1 min followed by substrate addition and measured after 30 mins by native polyacrylamide gel electrophoresis method relative to control	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
10.	ALA4418098	B	Inhibition of helicase activity of flag-tagged recombinant DNA2 D277A mutant (unknown origin) expressed in 293T cells assessed as remaining 5' to 3' end-dependent DNA helicase activity at 100 mM using M13 phage DNA hybridized to 5'-tailed oligonucleotide substrate and [g-32P]ATP pre-incubated with 10 nM DNA2 enzyme for 1 min followed by substrate addition and measured after 30 mins by native polyacrylamide gel electrophoresis method relative to control	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
11.	ALA4418099	B	Inhibition of helicase activity of flag-tagged recombinant DNA2 D277A mutant (unknown origin) expressed in 293T cells assessed as remaining 5' to 3' end-dependent DNA helicase activity at 100 mM using M13 phage DNA hybridized to 5'-tailed oligonucleotide substrate and [g-32P]ATP pre-incubated with 15 nM DNA2 enzyme for 1 min followed by substrate addition and measured after 30 mins by native polyacrylamide gel electrophoresis method relative to control	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
12.	ALA4418100	B	Inhibition of nuclease activity of flag-tagged recombinant DNA2 F696A mutant (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
13.	ALA4418101	B	Inhibition of nuclease activity of flag-tagged recombinant DNA2 L732A mutant (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
14.	ALA4418102	B	Binding affinity to flag-tagged recombinant DNA2 F696A mutant (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation using flap DNA substrate by EMSA	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
15.	ALA4418103	B	Binding affinity to flag-tagged recombinant DNA2 L732A mutant (unknown origin) expressed in 293T cells assessed as reduction in DNA2-substrate complex formation using flap DNA substrate by EMSA	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
16.	ALA4418104	B	Inhibition of nuclease activity of flag-tagged recombinant DNA2 (unknown origin) expressed in 293T cells using [32P]-labeled flap DNA substrate incubated for 15 mins by denaturing PAGE method	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
17.	ALA4418111	B	Inhibition of DNA2 in human U2OS cells assessed as reduction in single-strand annealing pathways by I-Scel/GFP-based reporter gene assay	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
18.	ALA4418112	B	Inhibition of DNA2 in human U2OS cells assessed as reduction in homologous recombination pathways by I-Scel/GFP-based reporter gene assay	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
19.	ALA4418113	B	Inhibition of DNA2 in human U2OS cells assessed as increase in gammaH2AX expression	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data
20.	ALA4418114	B	Inhibition of DNA2 in 150 nM CPT-treated human A549 cells assessed as inhibition of DNA replication fork restart by measuring restarting forks levels at 20 uM pre-incubated for 40 mins followed by incubation with 250 uM C1dU for 40 mins by DNA fiber assay (Rvb = 75%)	Homo sapiens	1	ALA4417634	cell-based format	Patent Bioactivity Data