# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4423907 | Binding | Inhibition of Gal4-fused TEAD2 (unknown origin) interaction with YAP expressed in human HeLa cells assessed as basal transcriptional activity level up to 100 uM after 6 hrs by nanoluciferase reporter gene assay | Homo sapiens | 1 | ALA4422642 | cell-based format | Scientific Literature | |
2. | ALA4423911 | Binding | Inhibition of Gal4-fused TEAD2 (unknown origin) interaction with YAP expressed in human HeLa cells assessed as basal transcriptional activity level after 6 hrs by nanoluciferase reporter gene assay | Homo sapiens | 1 | ALA4422642 | cell-based format | Scientific Literature | |
3. | ALA4670826 | Binding | Inhibition of CPM binding to nonacylated N-terminal His6-tagged human TEAD2 (217 to 447 residues) expressed in Escherichia coli BL21-CodonPlus (DE3)-RIPL cells preincubated for 10 mins followed by CPM addition and measured after 1 hr by fluorescence assay | Homo sapiens | 1 | ALA4665803 | cell-based format | Scientific Literature | |
4. | ALA5033516 | Binding | Binding affinity to eGFP-fused human TEAD2 (217 to 447 residues) expressed in CHO-K1 cells by microscale thermophoresis analysis | Homo sapiens | 3 | ALA5032518 | cell-based format | Scientific Literature | |
5. | ALA5033532 | Binding | Binding affinity to external surface of N-terminal His6-tagged human TEAD2 (217 to 447 residues) expressed in Escherichia coli BL21(DE3) assessed as melting temperature by Nano Differential scanning fluorometry | Homo sapiens | 1 | ALA5032518 | assay format | Scientific Literature | |
6. | ALA5105005 | Binding | Thermal stabilization of C-terminal human TEAD2 (217 to 447 residues) assessed as change in melting temperature by thermal shift assay | Homo sapiens | 1 | ALA5104107 | single protein format | Scientific Literature | |
7. | ALA5105007 | Binding | Binding affinity to recombinant TEAD2 (unknown origin) assessed as increase in thermal stability by thermal shift assay | Homo sapiens | 1 | ALA5104107 | single protein format | Scientific Literature | |
8. | ALA5149029 | Binding | Binding affinity to human TEAD2 (217 to 447 residues) expressed in Escherichia coli BL21 Star (DE3) by surface plasmon resonance assay | Homo sapiens | 2 | ALA5143725 | assay format | Scientific Literature | |
9. | ALA5149040 | Binding | Binding affinity to human TEAD2 (217 to 447 residues) expressed in Escherichia coli BL21 Star (DE3) assessed as change in melting temperature incubated for 2 hrs by measuring tryptophane fluorescence by Nano Differential scanning fluorometry | Homo sapiens | 7 | ALA5143725 | assay format | Scientific Literature | |
10. | ALA5149044 | Binding | Inhibition of pSF-CMV-P-FLAG tagged human TEAD2 palmitoylation transfected in HEK293 cells at 10 uM incubated for 6 hrs by Western blot analysis relative to control | Homo sapiens | 8 | ALA5143725 | cell-based format | Scientific Literature | |
11. | ALA5149049 | Binding | Binding affinity to human TEAD2 (217 to 447 residues) expressed in Escherichia coli BL21 Star (DE3) assessed as retention time by surface plasmon resonance assay | Homo sapiens | 1 | ALA5143725 | assay format | Scientific Literature | |
12. | ALA5149117 | Binding | Binding affinity to human TEAD2 (217 to 447 residues) expressed in Escherichia coli BL21 Star (DE3) assessed as change in melting temperature incubated for 2 hrs measuring scattering fluorescence by Nano Differential scanning fluorometry | Homo sapiens | 2 | ALA5143725 | assay format | Scientific Literature | |
13. | ALA5163789 | Binding | Displacement of FAM- labeled YAP1 peptide from TEAD2 (217 to 447 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) assessed as inhibition constant incubated for 1.5 hrs by fluorescence polarization-based competitive binding assay | Homo sapiens | 1 | ALA5154839 | assay format | Scientific Literature |