#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4419732	B	Inhibition of GST-tagged human intersectin-1 (1227 to 1571 residues) RhoGEF-catalyzed guanine nucleotide-exchange reaction of human full length Cdc42 (1 to 191 residues) by measuring inhibition of the GDP dissociation from Cdc42 at 0.76 to 250000 nM by Alexa 647 fluorescence dye based GDP/GTP exchange assay	Homo sapiens	1	ALA4419173	single protein format	Patent Bioactivity Data
2.	ALA4419750	B	Binding affinity to GST-tagged human intersectin-1 RhoGEF at 1 to 100000 nM by microscale thermophoresis analysis	Homo sapiens	1	ALA4419173	single protein format	Patent Bioactivity Data
3.	ALA4421136	B	Inhibition of N-terminal 6x-His-tagged recombinant human ITSN1 DH and PH domain (1227 to 1571 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged Cdc42 (1 to 182 residues) at 10 uM by BODIPY-FL-GDP dissociation assay	Homo sapiens	1	ALA4420078	assay format	Patent Bioactivity Data
4.	ALA4421137	B	Inhibition of N-terminal 6x-His-tagged recombinant human ITSN1 DH and PH domain (1227 to 1571 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged Cdc42 (1 to 182 residues) at 20 uM by BODIPY-FL-GDP dissociation assay	Homo sapiens	1	ALA4420078	assay format	Patent Bioactivity Data