| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4419732 | B | Inhibition of GST-tagged human intersectin-1 (1227 to 1571 residues) RhoGEF-catalyzed guanine nucleotide-exchange reaction of human full length Cdc42 (1 to 191 residues) by measuring inhibition of the GDP dissociation from Cdc42 at 0.76 to 250000 nM by Alexa 647 fluorescence dye based GDP/GTP exchange assay | Homo sapiens | 1 | single protein format | Patent Bioactivity Data | ||
| 2. | ALA4419750 | B | Binding affinity to GST-tagged human intersectin-1 RhoGEF at 1 to 100000 nM by microscale thermophoresis analysis | Homo sapiens | 1 | single protein format | Patent Bioactivity Data | ||
| 3. | ALA4421136 | B | Inhibition of N-terminal 6x-His-tagged recombinant human ITSN1 DH and PH domain (1227 to 1571 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged Cdc42 (1 to 182 residues) at 10 uM by BODIPY-FL-GDP dissociation assay | Homo sapiens | 1 | assay format | Patent Bioactivity Data | ||
| 4. | ALA4421137 | B | Inhibition of N-terminal 6x-His-tagged recombinant human ITSN1 DH and PH domain (1227 to 1571 residues) expressed in Escherichia coli BL21 (DE3) assessed as reduction in 50 nM SOS1-mediated GDP/GTP nucleotide exchange upon 2 uM N-terminal-GST-tagged Cdc42 (1 to 182 residues) at 20 uM by BODIPY-FL-GDP dissociation assay | Homo sapiens | 1 | assay format | Patent Bioactivity Data |
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