# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4305800 | B | Inhibition of C-terminal recombinant human ClpP (57 to 277 amino acids) expressed in Escherichia coli Rosetta2 cells using Suc-Leu-Tyr-AMC as substrate incubated for 1 hr and measured for 90 mins by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
2. | ALA4305801 | B | Inhibition of C-terminal recombinant human ClpP expressed in Escherichia coli Rosetta2 (DE3) cells using Suc-Leu-Tyr-AMC as substrate incubated for 15 mins and measured for 90 mins by fluorescence assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
3. | ALA4305802 | B | Inhibition of human ClpP expressed in Escherichia coli SG1146 cells using N-acetyl-Trp-Leu-Ala-AMC as substrate incubated for 1 hr and measured at 20 secs time interval for 90 mins by fluorescence assay | Homo sapiens | 11 | cell-based format | Scientific Literature | ||
4. | ALA5167707 | B | Agonist activity at human CLpP (57 to 277 amino acids) expressed in Escherichia coli using fluorogenic peptide AC-WLA-AMC as substrate incubated for 10 mins by fluorescence based assay | Homo sapiens | 52 | assay format | Scientific Literature | ||
5. | ALA5167708 | B | Binding affinity to human CLpP (57 to 277 amino acids) expressed in Escherichia coli assessed as change in melting temperature at 100 uM incubated for 30 mins by DSF assay | Homo sapiens | 2 | assay format | Scientific Literature | ||
6. | ALA5167714 | B | Binding affinity to CLpP in human HCT-116 cells assessed as protein stabilization at 10 uM incubated for 30 mins followed by heating at 46 to 74 degree celcius for 3 mins CETSA based Western blot analysis | Homo sapiens | 2 | cell-based format | Scientific Literature | ||
7. | ALA5167715 | B | Binding affinity to human CLpP assessed as dissociation constant by ITC method | Homo sapiens | 2 | single protein format | Scientific Literature | ||
8. | ALA5167716 | B | Binding affinity to human CLpP assessed as change in entropy by ITC method | Homo sapiens | 2 | single protein format | Scientific Literature | ||
9. | ALA5167717 | B | Binding affinity to human CLpP assessed as change in enthalpy by ITC method | Homo sapiens | 2 | single protein format | Scientific Literature | ||
10. | ALA5167718 | B | Potency index, ratio of ONC201 Kd to test compound Kd for binding affinity to human CLpP | Homo sapiens | 1 | single protein format | Scientific Literature | ||
11. | ALA5167803 | B | Agonist activity at human CLpP (57 to 277 amino acids) expressed in Escherichia coli assessed as induction of casein degradation using FITC-labelled casein as substrate | Homo sapiens | 6 | assay format | Scientific Literature | ||
12. | ALA5223588 | B | Agonist activity at human recombinant CLpP (57 to 277 amino acids) expressed in Escherichia coli using fluorogenic peptide AC-WLA-AMC as substrate incubated for 10 mins by fluorescence based assay | Homo sapiens | 5 | assay format | Scientific Literature |