# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4418719 | Binding | Inhibition of RPE65 in bovine RPE microsomes assessed as reduction in 11-cis-retinol production pre-incubated at room temperature for 5 mins before all-trans-retinol addition and further incubated for 15 mins to 2 hrs in presence of CRALBP by normal phase HPLC analysis | Bos taurus | 36 | ALA4418488 | assay format | Patent Bioactivity Data | |
2. | ALA4420247 | Binding | Inhibition of RPE65 in bovine RPE microsomes assessed as reduction in isomero-hydrolase activity using all-trans-[3H]-retinol by normal phase HPLC with flow scintillation analysis | Bos taurus | 11 | ALA4420061 | assay format | Patent Bioactivity Data | |
3. | ALA4669200 | Binding | Potency index, ratio of (R)-MB001 EC50 to test compound EC50 for inhibition of RPE65 in bovine RPE microsomes assessed as reduction in 11-cis-retinol production using all-trans-retinol as substrate pre-incubated with enzyme for 5 mins followed by further incubation with all-trans-retinol for 1 hr by normal phase HPLC analysis | Bos taurus | 2 | ALA4665714 | microsome format | Scientific Literature | |
4. | ALA4669201 | Binding | Inhibition of RPE65 in bovine RPE microsomes assessed as reduction in 11-cis-retinol production using all-trans-retinol as substrate pre-incubated with enzyme for 5 mins followed by further incubation with all-trans-retinol for 1 hr by normal phase HPLC analysis | Bos taurus | 3 | ALA4665714 | microsome format | Scientific Literature | |
5. | ALA5032820 | Binding | Inhibition of RPE65 in bovine retinal pigment epithelium microsomes assessed as decrease of 11-cis-retinol using trans-retinol measured after 1 hr by normal phase HPLC analysis | Bos taurus | 18 | ALA5032497 | microsome format | Scientific Literature |