#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4421089	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in ERK1/2 phosphorylation at 20 to 100 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
2.	ALA4421090	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as effect on Ras-GTP levels at 20 to 50 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by GST-Raf1 effector domain pulldown assay	Mus musculus	1		assay format	Patent Bioactivity Data
3.	ALA4421091	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as effect on R-Ras-GTP levels at 20 to 50 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by GST-Raf1 effector domain pulldown assay	Mus musculus	1		assay format	Patent Bioactivity Data
4.	ALA4421092	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in Akt phosphorylation at 20 to 100 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
5.	ALA4421093	B	Inhibition of SOS1 in mouse NIH/3T3 cells assessed as reduction in EGFR phosphorylation at 20 to 100 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		cell-based format	Patent Bioactivity Data
6.	ALA4421094	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in cell proliferation at 20 to 50 uM incubated for 3 days and measured every 24 hrs by MTS assay	Mus musculus	1		assay format	Patent Bioactivity Data
7.	ALA4421095	B	Inhibition of SOS1 in overnight serum-starved mouse MEF assessed as reduction in cell proliferation at 10 to 40 uM incubated for 3 days by MTS assay	Mus musculus	1		cell-based format	Patent Bioactivity Data
8.	ALA4421096	B	Inhibition of SOS1 in overnight serum-starved mouse MEF assessed as reduction in ERK1/2 phosphorylation at 10 to 40 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		cell-based format	Patent Bioactivity Data
9.	ALA4421097	B	Inhibition of SOS1 in overnight serum-starved mouse MEF assessed as reduction in Akt phosphorylation at 10 to 40 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		cell-based format	Patent Bioactivity Data
10.	ALA4421098	B	Inhibition of SOS1 in overnight serum-starved and H-RasG12V transduced mouse MEF assessed as reduction in cell proliferation at 10 to 40 uM incubated for 3 days by MTS assay	Mus musculus	1		cell-based format	Patent Bioactivity Data
11.	ALA4421099	B	Inhibition of SOS1 in overnight serum-starved and H-RasG12V transduced mouse MEF assessed as reduction in ERK1/2 phosphorylation at 10 to 40 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		cell-based format	Patent Bioactivity Data
12.	ALA4421100	B	Inhibition of SOS1 in overnight serum-starved and H-RasG12V transduced mouse MEF assessed as reduction in Akt phosphorylation at 10 to 40 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		cell-based format	Patent Bioactivity Data
13.	ALA4421112	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as effect on Rac1-GTP levels at 20 to 50 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by GST-Raf1 effector domain pulldown assay	Mus musculus	1		assay format	Patent Bioactivity Data
14.	ALA4421125	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as effect on Ras-GTP levels at 20 to 40 uM pre-incubated for 16 hrs before EGF stimulation for 5 mins by GST-Raf1 effector domain pulldown assay	Mus musculus	1		assay format	Patent Bioactivity Data
15.	ALA4421126	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in ERK1/2 phosphorylation at 20 to 40 uM pre-incubated for 16 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
16.	ALA4421127	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in Akt phosphorylation at 20 to 40 uM pre-incubated for 16 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
17.	ALA4421128	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as effect on Ras-GTP levels at 20 to 40 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by GST-Raf1 effector domain pulldown assay	Mus musculus	1		assay format	Patent Bioactivity Data
18.	ALA4421129	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in ERK1/2 phosphorylation at 20 to 40 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
19.	ALA4421130	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in Akt phosphorylation at 20 to 40 uM pre-incubated for 2 hrs before EGF stimulation for 5 mins by Western blot analysis	Mus musculus	1		assay format	Patent Bioactivity Data
20.	ALA4421138	B	Inhibition of SOS1 in overnight serum-starved mouse NIH/3T3 cells assessed as reduction in cell proliferation at 20 to 60 uM incubated for 3 days and measured every 24 hrs by MTS assay	Mus musculus	1		assay format	Patent Bioactivity Data