| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4334314 | Binding | Inhibition of recombinant human C-terminal His/N-terminal FLAG-tagged TET2 (1129 to 2002 residues) expressed in baculovirus infected sf9 cells assessed as reduction in oxidation of methylated dsDNA at 100 uM after 2 hrs by chemiluminescence assay relative to control | Homo sapiens | 6 | ALA4330181 | cell-based format | Scientific Literature | |
| 2. | ALA4334316 | Binding | Inhibition of recombinant human C-terminal His/N-terminal FLAG-tagged TET2 (1129 to 2002 residues) expressed in baculovirus infected sf9 cells assessed as reduction in oxidation of methylated dsDNA after 2 hrs by chemiluminescence assay | Homo sapiens | 1 | ALA4330181 | cell-based format | Scientific Literature | |
| 3. | ALA4822191 | Binding | Inhibition of TET2 (unknown origin) | Homo sapiens | 2 | ALA4819102 | single protein format | Scientific Literature | |
| 4. | ALA4822193 | Binding | Inhibition of TET2 (unknown origin) at 10 mM in presence of alpha-KG relative to control | Homo sapiens | 1 | ALA4819102 | single protein format | Scientific Literature | |
| 5. | ALA4822194 | Binding | Inhibition of TET2 (unknown origin) at 50 mM in presence of alpha-KG relative to control | Homo sapiens | 1 | ALA4819102 | single protein format | Scientific Literature | |
| 6. | ALA4822200 | Binding | Inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as reduction in 5hmc level incubated for 2 hrs by ELISA | Homo sapiens | 8 | ALA4819102 | assay format | Scientific Literature | |
| 7. | ALA4822201 | Binding | Potency index, ratio of IC50 of NOG to IC50 of compound for inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as reduction in 5hmc level incubated for 2 hrs by ELISA | Homo sapiens | 3 | ALA4819102 | assay format | Scientific Literature | |
| 8. | ALA4822204 | Binding | Binding affinity to human recombinant TET2 (1099 to 1936) catalytic domain assessed as dissociation constant by micro-scale thermophoresis analysis | Homo sapiens | 4 | ALA4819102 | single protein format | Scientific Literature | |
| 9. | ALA4822205 | Binding | Reversible inhibition of recombinant human GST-tagged TET2 (1099 to 1936 residues) expressed in Escherichia coli BL21 (DE3)pLysS assessed as restoration of enzyme activity in presence of alpha-KG at 20 uM incubated for 2 hrs by ELISA | Homo sapiens | 1 | ALA4819102 | assay format | Scientific Literature | |
| 10. | ALA5107791 | Binding | Inhibition of N-terminal His6-tagged TET2 (unknown origin) expressed in Escherichia coli assessed as reduction in 5-methylcytosine oxidation by measuring 5-carboxylcytosine level by FRET assay | Homo sapiens | 2 | ALA5104223 | assay format | Scientific Literature | |
| 11. | ALA5107792 | Binding | Inhibition of N-terminal His6-tagged TET2 (unknown origin) expressed in Escherichia coli assessed as reduction in 5-methylcytosine oxidation preincubated for 30 mins followed by Fe(II) addition for 1 hrs and Msp1 addition and measured after 4 hrs using oligonucleotide as substrate by FRET assay | Homo sapiens | 1 | ALA5104223 | assay format | Scientific Literature | |
| 12. | ALA5107793 | Binding | Inhibition of N-terminal His6-tagged TET2 (unknown origin) expressed in Escherichia coli assessed as reduction in hmC and fC levels preincubated for 30 mins followed by Fe(II) addition and measured after 1 hrs using 5'-CACmCGGTG-3' palindromic oligonucleotide as substrate by MALDI-TOF MS analysis | Homo sapiens | 18 | ALA5104223 | assay format | Scientific Literature | |
| 13. | ALA5135968 | Binding | Inhibition of recombinant human TET2 catalytic domain assessed as reduction of 5hmC formation at 125 uM using hemi-methylated synthetic oligomer 5'-AATTAGAA[5meC]GCTCCTT-3' as substrate incubated for 30 mins by LC-MS method | Homo sapiens | 2 | ALA5131531 | single protein format | Scientific Literature | |
| 14. | ALA5135970 | Binding | Inhibition of recombinant human TET2 catalytic domain assessed as reduction of 5hmC formation at 125 uM using hemi-methylated synthetic oligomer 5'-AATTAGAA[5meC]GCTCCTT-3' as substrate incubated for 30 mins in presence of CuSO4 by LC-MS method | Homo sapiens | 1 | ALA5131531 | single protein format | Scientific Literature | |
| 15. | ALA5135975 | Binding | Inhibition of recombinant TET2 in human Hep3B cells assessed as reduction of 5hmC levels at 50 uM incubated for 48 hrs in presence of CuSO4 by dot blotting analysis | Homo sapiens | 1 | ALA5131531 | cell-based format | Scientific Literature | |
| 16. | ALA5135976 | Binding | Inhibition of recombinant TET2 in human Hep3B cells assessed as reduction of 5hmC formation at 50 uM incubated for 48 hrs in presence of CuSO4 by HPLC-ESI-MS/MS | Homo sapiens | 1 | ALA5131531 | cell-based format | Scientific Literature |
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