# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4418408 | B | Inhibition of untagged human USP30 catalytic domain (57 to 517 residues) expressed in Escherichia coli using TAMRA labeled ubiquitin peptide as substrate preincubated for 30 min followed by substrate addition and measured after 2 hrs by fluorescence polarization assay | Homo sapiens | 272 | assay format | Patent Bioactivity Data | ||
2. | ALA4418409 | B | Inhibition of human USP30 catalytic domain (57 to 517 residues) using ubiquitin-rhodamine 110 as substrate preincubated for 30 min followed by substrate addition and measured after 2 hrs by fluorescence intensity kinetic assay | Homo sapiens | 272 | single protein format | Patent Bioactivity Data | ||
3. | ALA4418444 | B | Inhibition of untagged human USP30 catalytic domain (57 to 517 residues) expressed in Escherichia coli using TAMRA labeled ubiquitin peptide as substrate preincubated for 30 min followed by substrate addition and measured after 2 hrs by fluorescence polarization assay | Homo sapiens | 78 | assay format | Patent Bioactivity Data | ||
4. | ALA4418459 | B | Inhibition of untagged human USP30 catalytic domain (57 to 517 residues) expressed in Escherichia coli using TAMRA labeled ubiquitin peptide as substrate preincubated for 30 min followed by substrate addition and measured after 2 hrs by fluorescence polarization assay | Homo sapiens | 67 | assay format | Patent Bioactivity Data | ||
5. | ALA4418460 | B | Inhibition of untagged human USP30 catalytic domain (57 to 517 residues) expressed in Escherichia coli using TAMRA labeled ubiquitin peptide as substrate preincubated for 30 min followed by substrate addition and measured after 2 hrs by fluorescence polarization assay | Homo sapiens | 27 | assay format | Patent Bioactivity Data | ||
6. | ALA4418461 | B | Inhibition of untagged human USP30 catalytic domain (57 to 517 residues) expressed in Escherichia coli using TAMRA labeled ubiquitin peptide as substrate preincubated for 30 min followed by substrate addition and measured after 2 hrs by fluorescence polarization assay | Homo sapiens | 77 | assay format | Patent Bioactivity Data | ||
7. | ALA4418477 | B | Inhibition of USP30 (unknown origin) using ubiquitin-rhodamine 110 as substrate preincubated for 15 mins followed by substrate addition and measured after 2 hrs by fluorescence based assay | Homo sapiens | 90 | single protein format | Patent Bioactivity Data | ||
8. | ALA4605986 | B | Inhibition of human USP30 CD (57 to 517 residues) expressed in Escherichia coli assessed as cleavage of Ubiquitin-Rhodamine110-glycine to Ubiquitin and Rhodamine110-glycine using Ubiquitin-Rhodamine110-glycine as substrate by fluorescence based analysis | Homo sapiens | 1 | assay format | Scientific Literature | ||
9. | ALA4674624 | B | Inhibition of USP30 (unknown origin) | Homo sapiens | 2 | single protein format | Scientific Literature | ||
10. | ALA4674628 | B | Inhibition of USP30 (unknown origin) using Ubiquitin-Rhodamine110-Glycine as substrate incubated for 15 mins followed by substrate addition and measured after 2 hrs by fluorescence based assay | Homo sapiens | 48 | single protein format | Scientific Literature | ||
11. | ALA5108414 | B | Inhibition of GST-tagged recombinant USP30 (unknown origin) using Ub-Lys-TAMRA as substrate at 1 uM incubated for 30 mins by fluorescence polarization assay | Homo sapiens | 2 | single protein format | Scientific Literature | ||
12. | ALA5223299 | B | Inhibition of USP30 (unknown origin) | Homo sapiens | 1 | single protein format | Scientific Literature | ||
13. | ALA5251107 | B | Inhibition of human USP30 (57 to 517 residues) expressed in Sf9 cells using ubiquitin rhodamine 110 as substrate assessed as remaining enzyme activity at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | cell-based format | Scientific Literature | ||
14. | ALA5251142 | B | Inhibition of human USP30 using ubiquitin rhodamine 110 as substrate at 10 to 50 uM by fluorometric assay | Homo sapiens | 1 | single protein format | Scientific Literature | ||
15. | ALA5251164 | B | Inhibition of USP30 (unknown origin) using ubiquitin rhodamine 110 as substrate at 1 to 10 uM by DUBprofiler fluorometric assay | Homo sapiens | 2 | single protein format | Scientific Literature |