| # | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
|---|---|---|---|---|---|---|---|---|---|
| 1. | ALA4340697 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as succinate production at 500 uM incubated for 60 s in presence of 2OG and L-ascorbate and using Fe2 as cofactor by 1H NMR spectroscopy | Paramecium bursaria Chlorella virus 1 | 2 | cell-based format | Scientific Literature | ||
| 2. | ALA4340698 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as succinate production at 500 uM incubated for 60 s in presence of 2OG and in absence of L-ascorbate and using Fe2 as cofactor by 1H NMR spectroscopy | Paramecium bursaria Chlorella virus 1 | 2 | cell-based format | Scientific Literature | ||
| 3. | ALA4340699 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate hydroxylation at 100 uM incubated for 16 hrs in presence of 2OG by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 21 | cell-based format | Scientific Literature | ||
| 4. | ALA4340700 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate Kcat incubated for 16 hrs in presence of 2OG by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 1 | cell-based format | Scientific Literature | ||
| 5. | ALA4340701 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate Km incubated for 16 hrs in presence of 2OG by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 1 | cell-based format | Scientific Literature | ||
| 6. | ALA4340702 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate hydroxylation up to 1 mM incubated for 16 hrs in presence of 2OG by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 1 | cell-based format | Scientific Literature | ||
| 7. | ALA4340703 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate hydroxylation incubated for 16 hrs in presence of 2OG by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 1 | cell-based format | Scientific Literature | ||
| 8. | ALA4340704 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as substrate hydroxylation incubated for 16 hrs in presence of 2OG and in absence of L-ascorbate by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 1 | cell-based format | Scientific Literature | ||
| 9. | ALA4340705 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as Km(app) for Fe2 incubated for 16 hrs in presence of 2OG and Fe2 by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 2 | cell-based format | Scientific Literature | ||
| 10. | ALA4340706 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as Km for 2OG incubated for 16 hrs in presence of 2OG and Fe2 by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 2 | cell-based format | Scientific Literature | ||
| 11. | ALA4340707 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as succinate production at 500 uM incubated for 60 s in presence of 2OG and using Fe2 as cofactor by 1H NMR spectroscopy | Paramecium bursaria Chlorella virus 1 | 1 | cell-based format | Scientific Literature | ||
| 12. | ALA4340708 | B | Inhibition of N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells pre-incubated for 5 mins before 2OG as substrate and Fe2 as co-factor addition in presence of L-ascorbate and measured after 5 mins MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 28 | cell-based format | Scientific Literature | ||
| 13. | ALA4340713 | B | Substrate activity at N-terminal His6-tagged recombinant Paramecium bursaria chlorella virus 1 CPH expressed in Escherichia coli Rosetta 2 (DE3) cells assessed as Km(app) for ascorbate incubated for 16 hrs in presence of ascorbate, 2OG and Fe2 by MALDI TOF MS analysis | Paramecium bursaria Chlorella virus 1 | 2 | cell-based format | Scientific Literature |
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