# | Aladdin ID | Assay Type | Description | Organism | Compounds | Reference | BAO Format | Source | |
---|---|---|---|---|---|---|---|---|---|
1. | ALA4418419 | Binding | Inhibition of N-terminal 6xHis-SUMO tagged human HTRA1 catalytic-PDZ domain (D161 to P480 residues) expressed in Escherichia coli BL21 (DE3) cells using Mca-Ile-Arg-Arg-Val-Ser-Tyr-Ser-Phe-Lys(Dnp)-Lys as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by fluorescence method | Homo sapiens | 61 | ALA4418289 | cell-based format | Patent Bioactivity Data | |
2. | ALA4418436 | Binding | Inhibition of full length His-tagged human HTRA1 using Mca-H20PT as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by spectrofluorometry | Homo sapiens | 15 | ALA4418301 | single protein format | Patent Bioactivity Data | |
3. | ALA4418457 | Binding | Inhibition of N-terminal 6xHis-SUMO tagged human HTRA1 catalytic PDZ domain (D161 to P480 residues) expressed in Escherichia coli BL21 (DE3) cells using Mca-Ile-Arg-Arg-Val-Ser-Tyr-Ser-Phe-Lys(Dnp)-Lys as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by fluorescence method | Homo sapiens | 22 | ALA4418310 | cell-based format | Patent Bioactivity Data | |
4. | ALA4418458 | Binding | Inhibition of N-terminal 6xHis-SUMO tagged human HTRA1 catalytic PDZ domain (D161 to P480 residues) expressed in Escherichia coli BL21 (DE3) cells using Mca-Ile-Arg-Arg-Val-Ser-Tyr-Ser-Phe-Lys(Dnp)-Lys as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by fluorescence method | Homo sapiens | 23 | ALA4418311 | cell-based format | Patent Bioactivity Data | |
5. | ALA4422578 | Binding | Inhibition of N-terminal 6xHis-SUMO tagged human HTRA1 catalytic and PDZ domain (D161 to P480 residues) expressed in Escherichia coli BL21 (DE3) cells using Mca-Ile-Arg-Arg-Val-Ser-Tyr-Ser-Phe-Lys(Dnp)-Lys as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by fluorescence method | Homo sapiens | 17 | ALA4421735 | cell-based format | Patent Bioactivity Data | |
6. | ALA4477149 | Binding | Inhibition of full length His-tagged human HTRA1 using Mca-H20PT as substrate preincubated for 10 mins followed by substrate addition and measured after 10 mins by spectrofluorometry | Homo sapiens | 48 | ALA4476886 | single protein format | Patent Bioactivity Data | |
7. | ALA4482660 | Binding | Inhibition of His-tagged L56/HtrA1 (140 to 480 residues) (unknown origin) expressed in TB1 Escherichia coli at 10 to 50 uM pre-incubated for 15 mins before FITC-labeled dephosphorylated casein substrate addition and measured every 5 mins for 30 mins by fluorescence assay | Homo sapiens | 1 | ALA4482313 | assay format | Patent Bioactivity Data | |
8. | ALA5053474 | Binding | Inhibition of N-terminal 6xHis-SUMO tagged human HTRA1 catalytic PDZ domain (D161 to P480 residues) expressed in Escherichia coli BL21 (DE3) cells using Mca-Ile-Arg-Arg-Val-Ser-Tyr-Ser-Phe-Lys(Dnp)-Lys as substrate preincubated for 30 mins followed by substrate addition and measured after 2 hrs by fluorescence method | Homo sapiens | 205 | ALA5053470 | cell-based format | Patent Bioactivity Data | |
9. | ALA5258708 | Binding | Binding affinity to recombinant human HTRA1 assessed as increase in fluorescence using H2-OPT as substrate at 1 uM by SDS-PAGE based gel fluorescence scanning analysis | Homo sapiens | 9 | ALA5257069 | single protein format | Scientific Literature | |
10. | ALA5258714 | Binding | Binding affinity to human HTRA1 in human HeLa cells assessed as appearance of fluorescent bands at 5 uM by SDS-PAGE based gel fluorescence scanning analysis | Homo sapiens | 14 | ALA5257069 | cell-based format | Scientific Literature | |
11. | ALA5258718 | Binding | Inhibition of recombinant human HTRA1 using H2-OPT as substrate assessed as increase in fluorescence and measured for 2 hrs by fluorescent plate reader analysis | Homo sapiens | 10 | ALA5257069 | single protein format | Scientific Literature | |
12. | ALA5258720 | Binding | Inhibition of recombinant human HTRA1 using H2-OPT as substrate assessed as increase in fluorescence at 1 uM and measured for 2 hrs by fluorescent plate reader analysis | Homo sapiens | 1 | ALA5257069 | single protein format | Scientific Literature | |
13. | ALA5258722 | Binding | Binding affinity to human HTRA1 in human U-87 MG cells assessed as appearance of fluorescent bands at 5 uM by SDS-PAGE based In-gel fluorescence analysis | Homo sapiens | 2 | ALA5257069 | cell-based format | Scientific Literature | |
14. | ALA5258724 | Binding | Binding affinity to human HTRA1 in Amyloid-beta treated human U-87 MG cells assessed as appearance of fluorescent bands at 5 uM by SDS-PAGE based In-gel fluorescence analysis | Homo sapiens | 1 | ALA5257069 | cell-based format | Scientific Literature | |
15. | ALA5258726 | Binding | Binding affinity to recombinant human HTRA1 in human serum assessed as appearance of fluorescent bands at 2 uM by In-gel fluorescence analysis | Homo sapiens | 2 | ALA5257069 | cell-free format | Scientific Literature |