#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4358853	B	Inhibition of recombinant human His6-Otubain-1 assessed as reduction in cleavage of luminescent substrate Ub-AML at 100 uM	Homo sapiens	1	ALA4354861	single protein format	Scientific Literature
2.	ALA4372627	B	Binding affinity to OTUB1 in human HeLa cell lysates assessed as cysteine-compound adduct formation at 50 uM preincubated for 22 mins followed by IAA-probe addition and measured after 20 mins by mass spectrometric method	Homo sapiens	1	ALA4371052	subcellular format	Scientific Literature
3.	ALA5112518	B	Destabilization of N-terminal His-6 tagged OTUB1 (40 to 271 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells assessed as reduction in melting temperature at 200 uM measured for 2 hrs in presence of dithiothreitol by SYPRO orange dye based thermal shift assay	Homo sapiens	4	ALA5108067	cell-based format	Scientific Literature
4.	ALA5112519	B	Inhibition of N-terminal His-6 tagged OTUB1 (40 to 271 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells at 10 nM using Ub-Rho measured after 0.5 to 3 hrs by fluorescence based Plate reader method relative to control	Homo sapiens	68	ALA5108067	cell-based format	Scientific Literature
5.	ALA5112520	B	Inhibition of N-terminal His-6 tagged OTUB1 (40 to 271 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells at 100 nM using Ub-Rho measured after 0.5 to 3 hrs by fluorescence based Plate reader method relative to control	Homo sapiens	68	ALA5108067	cell-based format	Scientific Literature
6.	ALA5112525	B	Inhibition of N-terminal His-6 tagged OTUB1 (40 to 271 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells using Ub-Rho measured after 0.5 to 3 hrs by fluorescence based Plate reader method	Homo sapiens	6	ALA5108067	cell-based format	Scientific Literature
7.	ALA5112532	B	Inhibition of N-terminal His-6 tagged OTUB1 (40 to 271 residues) (unknown origin) expressed in Escherichia coli BL21 (DE3) cells at 2.5 uM using Ub-Rho measured after 0.5 to 3 hrs by fluorescence based Plate reader method relative to control	Homo sapiens	2	ALA5108067	cell-based format	Scientific Literature
8.	ALA5112538	B	Inhibition of OTUB1 in human NCI-H1975 cells assessed as reduction in protein level at 500 nM measured for 24 hrs by immunoblotting analysis	Homo sapiens	1	ALA5108067	cell-based format	Scientific Literature
9.	ALA5112555	B	Binding affinity to OTUB1 in HEK293T cells at 2.5 uM preincubated for 30 mins followed by UV irradiation and measured after 20 mins by Western blot analysis	Homo sapiens	1	ALA5108067	cell-based format	Scientific Literature
10.	ALA5112556	B	Binding affinity to OTUB1 in HEK293T cells at 5 uM preincubated for 30 mins followed by UV irradiation and measured after 20 mins by Western blot assay	Homo sapiens	1	ALA5108067	cell-based format	Scientific Literature
11.	ALA5112557	B	Binding affinity to OTUB1 in HEK293T cells at 1 uM preincubated for 2 hrs followed by UV irradiation and measured after 20 mins by silver staining based gel electrophoresis assay	Homo sapiens	1	ALA5108067	cell-based format	Scientific Literature
12.	ALA5112558	B	Binding affinity to OTUB1 in HEK293T cells at 0.5 uM preincubated for 2 hrs followed by UV irradiation and measured after 20 mins by Western blot analysis	Homo sapiens	1	ALA5108067	cell-based format	Scientific Literature
13.	ALA5112559	B	Inhibition of OTUB1 in human NCI-H1975 cells assessed as reduction in UBE2N protein level at 500 nM measured for 24 hrs by immunoblotting analysis	Homo sapiens	2	ALA5108067	cell-based format	Scientific Literature