#	Aladdin ID	Assay Type	Description	Organism	Compounds	Reference	BAO Format	Source
1.	ALA4432926	B	Binding affinity to recombinant hexa-histidine-tagged human full-length N-GLY1 catalytic domain expressed in Escherichia coli BL21 (DE3) assessed as change in melting temperature by thermal shift assay	Homo sapiens	9	ALA4431380	assay format	Scientific Literature
2.	ALA4432927	B	Binding affinity to recombinant hexa-histidine-tagged human full-length N-GLY1 catalytic domain expressed in Escherichia coli BL21 (DE3) by thermal shift assay	Homo sapiens	9	ALA4431380	assay format	Scientific Literature
3.	ALA5053072	B	Inhibition of NGLY1 in human HEK293T cells at 200 uM assessed as retention of N-glycans on full-length NFE2L1 measured by electrophoretic mobility shift assay	Homo sapiens	1	ALA5052832	cell-based format	Patent Bioactivity Data
4.	ALA5053077	B	Inhibition of NGLY1 in human UACC-257 cells at 200 uM assessed as increase in IFN-beta1 production measured after 48 hrs	Homo sapiens	3	ALA5052832	cell-based format	Patent Bioactivity Data
5.	ALA5053078	B	Inhibition of NGLY1 in human UACC-257 cells at 200 uM assessed as increase in IL-29 production measured after 48 hrs	Homo sapiens	3	ALA5052832	cell-based format	Patent Bioactivity Data
6.	ALA5053079	B	Inhibition of NGLY1 in human SK-MEL-2 cells at 200 uM assessed as increase in IFN-beta1 production measured after 48 hrs	Homo sapiens	3	ALA5052832	cell-based format	Patent Bioactivity Data
7.	ALA5053080	B	Inhibition of NGLY1 in human SK-MEL-2 cells at 200 uM assessed as increase in IL-29 production measured after 48 hrs	Homo sapiens	3	ALA5052832	cell-based format	Patent Bioactivity Data
8.	ALA5053081	B	Inhibition of NGLY1 in human HDF51 cells at 200 uM assessed as increase in IFN-beta1 production measured after 48 hrs	Homo sapiens	2	ALA5052832	cell-based format	Patent Bioactivity Data
9.	ALA5053082	B	Inhibition of NGLY1 in human HDF51 cells at 200 uM assessed as increase in IL-29 production measured after 48 hrs	Homo sapiens	2	ALA5052832	cell-based format	Patent Bioactivity Data